ArticleCell & bioscience2022
Single cell transcriptomic analysis of human amnion identifies cell-specific signatures associated with membrane rupture and parturition.
Article in Cell & bioscience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 33 citations in OpenAlex.
- Spatial Logic and Evolutionary Innovation in Human Placentation.bioRxiv : the preprint server for biology · 2026Article
- Recent advances on anti-scarring properties of amniotic membrane: emerging strategies and clinical potentials in regenerative medicine.Stem cell research & therapy · 2026Review
- Ex vivo model for assessing fetal membrane integrity and therapeutic strategies.Scientific reports · 2026Article
- M2 macrophages undergoing ferroptosis regulate uterine smooth muscle contraction to facilitate labor onset.Scientific reports · 2025Article
- Chemokines in Pregnancy.Biomolecules · 2025Review
- Conservation of gene expression patterns between the amniotic and nasal epithelium at birth.ERJ open research · 2025Article
- Atlas of amnion development during the first trimester of human pregnancy.Nature cell biology · 2025Article
- The maternal-fetal interface at single-cell resolution: uncovering the cellular anatomy of the placenta and decidua.American journal of obstetrics and gynecology · 2025Review
- Can activator protein-1 transcription factors be monitored in the maternal circulation to predict set on labor?Obstetrics & gynecology science · 2025Article
- Review
- ADAMTS4 is a crucial proteolytic enzyme for versican cleavage in the amnion at parturition.Communications biology · 2024Article
- Deciphering maternal-fetal cross-talk in the human placenta during parturition using single-cell RNA sequencing.Science translational medicine · 2024Article
- Spatial transcriptomics of fetal membrane-Decidual interface reveals unique contributions by cell types in term and preterm births.PloS one · 2024Article
- Integrated single-nucleus sequencing and spatial architecture analysis identified distinct injured-proximal tubular types in calculi rats.Cell & bioscience · 2023Article
- Amnion-derived serum amyloid A1 participates in sterile inflammation of fetal membranes at parturition.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2023Article
- Editorial: Advance in translational research of preterm birth and related pregnancy.Frontiers in pharmacology · 2022Article
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Authors and funding
11 authors at 3 institutions in 2 countries.
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Abstract
backgroundThe human amnion is an intrauterine tissue which is involved in the initiation of parturition. In-depth understanding of gene expression signatures of individual cell types in the amnion with respect to membrane rupture at parturition may help identify crucial initiators of parturition for the development of specific strategies to prevent preterm birth, a leading cause of perinatal mortality.
resultsSix major cell types were revealed in human amnion including epithelial cells, fibroblasts and immunocytes as well as three other cell types expressing dual cell markers including epithelial/fibroblast, immune/epithelial and immune/fibroblast markers. The existence of cell types expressing these dual cell markers indicates the presence of epithelial-mesenchymal (EMT), epithelial-immune (EIT) and mesenchymal-immune (MIT) transitions in amnion at parturition. We found that the rupture zone of amnion exhibited some specific increases in subcluster proportions of immune and EMT cells related to extracellular matrix remodeling and inflammation in labor. The non-rupture zone exhibited some common changes in subcluster compositions of epithelial and fibroblast cells with the rupture zone in labor, particularly those related to oxidative stress and apoptosis in epithelial cells and zinc ion transport in fibroblasts. Moreover, we identified that C-C motif chemokine ligand 20 (CCL20) was among the top up-regulated genes in amnion epithelial cells, fibroblasts and immunocytes in the rupture zone at parturition. Studies in pregnant mice showed that administration of CCL20 induced immunocytes infiltration to tissues at the maternal-fetal interface and led to preterm birth.
conclusionsApart from the conventional epithelial, fibroblast and immunocytes, human amnion cells may undergo EMT, EIT and FIT in preparation for parturition. Intense inflammation and ECM remodeling are present in the rupture zone, while enhanced apoptosis and oxidative stress in epithelial cells and zinc ion transport in fibroblasts are present in amnion regardless of the rupture zones at parturition. CCL20 derived from the major cell types of the amnion participates in labor onset.
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