Evidence map›Paper›PMID 35599315›Full record

Trial reportGenome medicine2022

Gut microbiota predicts body fat change following a low-energy diet: a PREVIEW intervention study.

Ching Jian, Marta Paulino Silvestre, Danielle Middleton, Katri Korpela, Elli Jalo, David Broderick, Willem Meindert de Vos, Mikael Fogelholm, Mike William Taylor, Anne Raben and 2 more

Registry-linked trialOpen access · goldAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Genome medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01777893 (PREVention of Diabetes Through Lifestyle Intervention and Population Studies in Europe and Around the World), which is not on this map. Cited by 63 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
63citing papers in PubMed, 4 pooled it
8.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT01777893 nacompletednot on this map

PREVention of Diabetes Through Lifestyle Intervention and Population Studies in Europe and Around the World

TypeinterventionalSponsorAnne Birgitte RabenRan2013 to 2018Enrolled2,500ConditionsPre-diabetes, ObesityArmsHigh protein/ high intensity physical activity (HP-HI), High protein / moderate intensity physical activity (HP-MI), Moderate protein/ high intensity physical activity (MP-HI), Moderate protein/ moderate intensity physical activity (MP-MI)
3 · Its place in the literature

Who cites it

63 citing papers in PubMed, 4 syntheses or guidelines pooled it, 74 citations in OpenAlex.

  1. Pooled it
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  3. Roles of the gut microbiome in weight management.Nature reviews. Microbiology · 2023
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  6. Testosterone administration partially modulates gut microbiota responses to severe energy deficit.American journal of physiology. Endocrinology and metabolism · 2026
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3 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 4 countries.

Ching Jian *Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland.ORCID 0000-0003-0577-8834
Marta Paulino Silvestre *NOVA Medical School, NOVA University of Lisbon | Centro de Investigação em Tecnologias e Serviços de Saúde (CINTESIS), Lisbon, Portugal.
Danielle MiddletonSchool of Biological Sciences, University of Auckland, Auckland, New Zealand.
Katri KorpelaHuman Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland.
Elli JaloDepartment of Food and Nutrition, Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, Finland.
David BroderickSchool of Biological Sciences, University of Auckland, Auckland, New Zealand.
Willem Meindert de VosHuman Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland.
Mikael FogelholmDepartment of Food and Nutrition, Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, Finland.
Mike William TaylorSchool of Biological Sciences, University of Auckland, Auckland, New Zealand.
Anne RabenDepartment of Nutrition, Exercise and Sports, University of Copenhagen and Steno Diabetes Center, Copenhagen, Denmark.
Sally PoppittHuman Nutrition Unit, School of Biological Sciences, Department of Medicine, University of Auckland, Auckland, New Zealand.
Anne SalonenHuman Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland. anne.salonen@helsinki.fi.ORCID 0000-0002-6960-7447
University of Helsinki · FIUniversity of Auckland · NZUniversity of Copenhagen · DKUniversity of Lisbon · PT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundLow-energy diets (LEDs) comprise commercially formulated food products that provide between 800 and 1200 kcal/day (3.3-5 MJ/day) to aid body weight loss. Recent small-scale studies suggest that LEDs are associated with marked changes in the gut microbiota that may modify the effect of the LED on host metabolism and weight loss. We investigated how the gut microbiota changed during 8 weeks of total meal replacement LED and determined their associations with host response in a sub-analysis of 211 overweight adults with pre-diabetes participating in the large multicentre PREVIEW (PREVention of diabetes through lifestyle intervention and population studies In Europe and around the World) clinical trial.

methodsMicrobial community composition was analysed by Illumina sequencing of the hypervariable V3-V4 regions of the 16S ribosomal RNA (rRNA) gene. Butyrate production capacity was estimated by qPCR targeting the butyryl-CoA:acetate CoA-transferase gene. Bioinformatics and statistical analyses, such as comparison of alpha and beta diversity measures, correlative and differential abundances analysis, were undertaken on the 16S rRNA gene sequences of 211 paired (pre- and post-LED) samples as well as their integration with the clinical, biomedical and dietary datasets for predictive modelling.

resultsThe overall composition of the gut microbiota changed markedly and consistently from pre- to post-LED (P = 0.001), along with increased richness and diversity (both P < 0.001). Following the intervention, the relative abundance of several genera previously associated with metabolic improvements (e.g., Akkermansia and Christensenellaceae R-7 group) was significantly increased (P < 0.001), while flagellated Pseudobutyrivibrio, acetogenic Blautia and Bifidobacterium spp. were decreased (all P < 0.001). Butyrate production capacity was reduced (P < 0.001). The changes in microbiota composition and predicted functions were significantly associated with body weight loss (P < 0.05). Baseline gut microbiota features were able to explain ~25% of variation in total body fat change (post-pre-LED).

conclusionsThe gut microbiota and individual taxa were significantly influenced by the LED intervention and correlated with changes in total body fat and body weight in individuals with overweight and pre-diabetes. Despite inter-individual variation, the baseline gut microbiota was a strong predictor of total body fat change during the energy restriction period.

trial registrationThe PREVIEW trial was prospectively registered at ClinicalTrials.gov ( NCT01777893 ) on January 29, 2013.

Indexed as

Gastrointestinal MicrobiomePrediabetic StateAdipose TissueAdultButyratesDietFecesHumansOverweightRNA, Ribosomal, 16SWeight LossButyratesRNA, Ribosomal, 16S

Identifiers

PMID35599315
PMCPMC9125896
OpenAlexW4281291801

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.