Evidence map›Paper›PMID 35601142›Full record

ArticleBioMed research international2022

Production Optimization and Biochemical Characterization of Cellulase from

Subash Khadka, Deegendra Khadka, Ram Chandra Poudel, Mukund Bhandari, Purnima Baidya, Jaishree Sijapati, Jyoti Maharjan

Open access · hybridAbstract read
In one paragraph

Article in BioMed research international, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.7field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 27 citations in OpenAlex.

  1. Characterization of crude cellulase produced by Bacillus licheniformis OE-1 from the Diyadin hot springs, Ağrı Province.International microbiology : the official journal of the Spanish Society for Microbiology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Subash KhadkaMolecular Biotechnology Unit, Nepal Academy of Science and Technology, Lalitpur, Nepal.ORCID https://orcid.org/0000-0001-7982-9476
Deegendra KhadkaMolecular Biotechnology Unit, Nepal Academy of Science and Technology, Lalitpur, Nepal.ORCID https://orcid.org/0000-0002-2838-1277
Ram Chandra PoudelMolecular Biotechnology Unit, Nepal Academy of Science and Technology, Lalitpur, Nepal.ORCID https://orcid.org/0000-0002-6603-0822
Mukund BhandariDepartment of Molecular Medicine, University of Texas Health Science Center at San Antonio, Texas, USA.ORCID https://orcid.org/0000-0001-9648-4844
Purnima BaidyaCentral Department of Microbiology, Tribhuvan University, Kathmandu, Nepal.
Jaishree SijapatiMolecular Biotechnology Unit, Nepal Academy of Science and Technology, Lalitpur, Nepal.ORCID https://orcid.org/0000-0002-1481-7316
Jyoti MaharjanMolecular Biotechnology Unit, Nepal Academy of Science and Technology, Lalitpur, Nepal.ORCID https://orcid.org/0000-0003-3671-9853
Nepal Academy of Science and Technology · NPTribhuvan University · NPThe University of Texas Health Science Center at San Antonio · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study is aimed at isolating and identifying a thermophilic cellulolytic bacterium from hot spring water and characterizing thermostable cellulase produced by the isolate. Enrichment and culture of water sample was used for isolation of bacterial strains and an isolate with highest cellulase activity was chosen for the production, partial purification, and biochemical characterization of the enzyme. Different staining techniques, enzymatic tests, and 16s ribosomal DNA (16s rDNA) gene sequencing were used for the identification of the isolate. The cellulase producing isolate was Gram positive, motile, and sporulated rod-shaped bacterium growing optimally between 55°C and 65°C. Based on partial 16s rDNA sequence analysis, the isolate was identified as

Indexed as

CellulaseGeobacillusHot SpringsDNA, RibosomalEnzyme StabilityHot TemperatureHydrogen-Ion ConcentrationNepalPhylogenyTemperatureWaterCellulaseDNA, RibosomalWater

Identifiers

PMID35601142
PMCPMC9119783
OpenAlexW4280637252

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.