Evidence map›Paper›PMID 35612284›Full record

ArticlemBio2022

The Tetraspanin CD81 Is a Host Factor for Chikungunya Virus Replication.

Lisa Lasswitz, Francisco J Zapatero-Belinchón, Rebecca Moeller, Kirsten Hülskötter, Timothée Laurent, Lars-Anders Carlson, Christine Goffinet, Graham Simmons, Wolfgang Baumgärtner, Gisa Gerold

Open access · goldAbstract read
In one paragraph

Article in mBio, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
3.6field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 19 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. The SMARCA4-TMEM47 axis plays an essential role in chikungunya virus RNA replication.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  5. Review
  6. Functional analysis ofThe Journal of general virology · 2025
    Article
  7. Infectomics of Chikungunya Virus: Roles Played by Host Factors.The American journal of tropical medicine and hygiene · 2025
    Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Towards modelling tick-virus interactions using the weakly pathogenicFrontiers in cellular and infection microbiology · 2024
    Article
  15. Article
  16. Review
  17. Review
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 3 countries.

Lisa LasswitzInstitute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hanover, Germany.ORCID 0000-0002-0393-076X
Francisco J Zapatero-BelinchónInstitute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hanover, Germany.
Rebecca MoellerInstitute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hanover, Germany.
Kirsten HülskötterDepartment of Pathology, University of Veterinary Medicine Hanover, Hanover, Germany.
Timothée LaurentWallenberg Centre for Molecular Medicine (WCMM), Umeå University, Umeå, Sweden.
Lars-Anders CarlsonWallenberg Centre for Molecular Medicine (WCMM), Umeå University, Umeå, Sweden.
Christine GoffinetCharité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Institute of Virology, Berlin, Germany.
Graham SimmonsVitalant Research Institute, University of California, San Francisco, California, USA.
Wolfgang BaumgärtnerDepartment of Pathology, University of Veterinary Medicine Hanover, Hanover, Germany.
Gisa GeroldInstitute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hanover, Germany.ORCID 0000-0002-1326-5038
University of Veterinary Medicine Hannover, Foundation · DEUmeå University · SEHumboldt-Universität zu Berlin · DEUniversity of California, San Francisco · US

Funding

Protective B-cell responses in chikungunya virus infectionR01AI119056 · NIAID · VITALANT · PI SIMMONS, GRAHAM · 2016 to 2019
$1.9M
NIAID NIH HHS R01 AI119056
6 · The paper itself

Abstract

Chikungunya virus (CHIKV) is an arthritogenic reemerging virus replicating in plasma membrane-derived compartments termed "spherules." Here, we identify the human transmembrane protein CD81 as host factor required for CHIKV replication. Ablation of CD81 results in decreased CHIKV permissiveness, while overexpression enhances infection. CD81 is dispensable for virus uptake but critically required for viral genome replication. Likewise, murine CD81 is crucial for CHIKV permissiveness and is expressed in target cells such as dermal fibroblasts, muscle and liver cells. Whereas related alphaviruses, including Ross River virus (RRV), Semliki Forest virus (SFV), Sindbis virus (SINV) and Venezuelan equine encephalitis virus (VEEV), also depend on CD81 for infection, RNA viruses from other families, such as coronaviruses, replicate independently of CD81. Strikingly, the replication-enhancing function of CD81 is linked to cholesterol binding. These results define a mechanism exploited by alphaviruses to hijack the membrane microdomain-modeling protein CD81 for virus replication through interaction with cholesterol.

Indexed as

Chikungunya FeverChikungunya virusVirusesAnimalsCholesterolHumansMiceTetraspaninsVirus ReplicationCholesterolTetraspaninsalphavirusarenavirusCD81chikungunya virusCHIKVcoronavirusreplicationtetraspanin

Identifiers

PMID35612284
PMCPMC9239085
OpenAlexW4281489874

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.