ArticleJournal of clinical laboratory analysis2022
Measurement comparability of insulin assays using conventional immunoassay kits.
Article in Journal of clinical laboratory analysis, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Hyperinsulinaemia as a cause of obesity and cardiometabolic diseases.Nature reviews. Endocrinology · 2026Review
- Age-Dependent Differences in Exercise Response Among Healthy Women: Impact on Inflammation, Lipids Profile and Glucose.Biomedicines · 2026Article
- A candidate reference measurement procedure for quantification of human insulin in serum based on immunoaffinity extraction and isotope dilution-liquid chromatography-tandem mass spectrometry.Analytical and bioanalytical chemistry · 2025Article
- Amino Acid Hepatotoxicity Biomarkers in Human Hepatic Organoids: Promising Standardization of Drug Toxicity Evaluation.ACS pharmacology & translational science · 2025Article
- Article
- A Fresh Look at Islet Isolation from Rabbit Pancreases.International journal of molecular sciences · 2024Article
- Aged garlic extract preserves beta-cell functioning via modulation of nuclear factor kappa-B (NF-κB)/Toll-like receptor (TLR)-4 and sarco endoplasmic reticulum calcium ATPase (SERCA)/CaDiabetology & metabolic syndrome · 2024Article
- A prospective guide for clinical implementation of selected OGTT- derived surrogate indices for the evaluation of β- cell function and insulin sensitivity in patients with transfusion-dependent β- thalassaemia.Acta bio-medica : Atenei Parmensis · 2023Article
- Review
- Label-free Aptasensor for the Ultrasensitive Detection of Insulin Via a Synergistic Fluorescent Turn-on Strategy Based on G-quadruplex and AIEgens.Journal of fluorescence · 2023Article
- Gamma-glutamyl transferase to high-density lipoprotein cholesterol ratio is a more powerful marker than TyG index for predicting metabolic syndrome in patients with type 2 diabetes mellitus.Frontiers in endocrinology · 2023Article
- Measurement comparability of insulin assays using conventional immunoassay kits.Journal of clinical laboratory analysis · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
backgroundThe standardization of measurement aims to achieve comparability of results regardless of the analytical methods and the laboratory where analyses are carried out. In this paper, a comparison of results from several immunoassay-based insulin analysis kits is described, and the steps necessary to improve comparability are discussed.
methodsFour manual enzyme-linked immunosorbent assay (ELISA) kits produced by Mercodia, Alpco, Epitope Diagnostics, and Abcam, and three automated chemiluminescent (CLIA) insulin assay kits (Siemens Centaur XP, Unicel Dxl800, Cobas e801) were compared by analyzing human serum samples and certified reference materials for human insulin.
resultsThe seven evaluated assay kits showed substantial discrepancies in the results, with relative standard deviation ranges between 1.7% and 23.2%. We find that the traceability chains and the unit conversion factors are not yet harmonized, and current reference materials for insulin are not applicable for immunoassay-based method validation due to the use of different matrices.
conclusionsThe findings suggest the need to fine tune insulin analysis methods, measurement traceability, and any conversion factor used in post-analysis steps in accordance with the necessity for standardization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.