Evidence map›Paper›PMID 35624329›Full record

ArticleDigestive diseases and sciences2022

Exosomes Derived from BMSCs Ameliorate Intestinal Ischemia-Reperfusion Injury by Regulating miR-144-3p-Mediated Oxidative Stress.

Guangru Zhang, Zhanhai Wan, Zhenzhen Liu, Disheng Liu, Zhiyu Zhao, Yufang Leng

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Digestive diseases and sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Guangru ZhangThe First School of Clinical Medicine, Lanzhou University, Lanzhou, 730000, China.ORCID 0000-0002-5875-7642
Zhanhai WanThe First School of Clinical Medicine, Lanzhou University, Lanzhou, 730000, China.ORCID 0000-0003-2521-207X
Zhenzhen LiuThe First School of Clinical Medicine, Lanzhou University, Lanzhou, 730000, China.ORCID 0000-0002-0387-3427
Disheng LiuThe First School of Clinical Medicine, Lanzhou University, Lanzhou, 730000, China.ORCID 0000-0002-5718-7377
Zhiyu ZhaoThe First School of Clinical Medicine, Lanzhou University, Lanzhou, 730000, China.ORCID 0000-0001-7067-6178
Yufang LengThe First School of Clinical Medicine, Lanzhou University, Lanzhou, 730000, China. lengyf@lzu.edu.cn.ORCID 0000-0001-7094-2413
First Hospital of Lanzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIntestinal ischemia-reperfusion (I/R) injury is a critical pathophysiological process involved in many acute and critical diseases, and it may seriously threaten the lives of patients. Exosomes derived from bone marrow mesenchymal stem cells (BMSC-exos) may be an effective therapeutic approach for I/R injury.

aimsThis study aimed to investigate the role and possible mechanism of BMSC-exos in intestinal I/R injury in vivo and in vitro based on the miR-144-3p and PTEN/Akt/Nrf2 pathways.

methodsBMSC-exos were isolated from mouse BMSCs by super centrifugation methods. The effects of BMSC-exos on I/R intestinal injury, intestinal cell apoptosis, oxidative stress and the PTEN/Akt/Nrf2 pathway were explored in vivo and in vitro. Furthermore, the relationship between miR-144-3p and PTEN was confirmed by a dual-luciferase reporter assay. The miR-144-3p mimic and inhibitor were used to further clarify the role of miR-144-3p in the mitigation of intestinal I/R by BMSC-exos.

resultsBMSC-exos effectively alleviated intestinal pathological injury, reduced intestinal cell apoptosis, relieved oxidative stress and regulated the PTEN/Akt/Nrf2 pathway in vivo and in vitro. In addition, miR-144-3p was significantly reduced in the oxygen and glucose deprivation/reperfusion cell model, and miR-144-3p could directly target PTEN to regulate its expression. Additional studies showed that changing the expression of miR-144-3p in BMSC-exos significantly affected the regulation of intestinal injury, intestinal cell apoptosis, oxidative stress and the PTEN/Akt/Nrf2 pathway in I/R, suggesting that miR-144-3p in BMSC-exos plays an important role in regulating the PTEN/Akt/Nrf2 during intestinal I/R.

conclusionsBMSC-exos carrying miR-144-3p alleviated intestinal I/R injury by regulating oxidative stress.

Indexed as

ExosomesMicroRNAsOxidative StressReperfusion InjuryAnimalsGlucoseMesenchymal Stem CellsMiceNF-E2-Related Factor 2OxygenProto-Oncogene Proteins c-aktGlucoseMicroRNAsMIRN144 microRNA, mouseNF-E2-Related Factor 2OxygenProto-Oncogene Proteins c-aktBMSCsExosomesIntestinal ischemia–reperfusion injurymiR-144-3pOxidative stressPTEN/Akt/Nrf2

Identifiers

PMID35624329
OpenAlexW4281850535

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.