Visualising the endothelial glycocalyx in dogs.

Sara J Lawrence-Mills, Chris R Neal, Simon C Satchell, Gavin I Welsh, Rebecca R Foster, Natalie Finch

Open access · hybridAbstract read

In one paragraph

Article in Veterinary journal (London, England : 1997), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.3field-weighted citation impact, top 45% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

The microvascular endothelial glycocalyx: An additional piece of the puzzle in veterinary medicine.Veterinary journal (London, England : 1997) · 2022
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Sara J Lawrence-MillsBristol Renal, Bristol Medical School, University of Bristol, Bristol, UK; The Royal Veterinary College, University of London, North Mymms, UK. Electronic address: slawrencemills21@rvc.ac.uk.

Chris R NealBristol Renal, Bristol Medical School, University of Bristol, Bristol, UK.

Simon C SatchellBristol Renal, Bristol Medical School, University of Bristol, Bristol, UK.

Gavin I WelshBristol Renal, Bristol Medical School, University of Bristol, Bristol, UK.

Rebecca R FosterBristol Renal, Bristol Medical School, University of Bristol, Bristol, UK.

Natalie FinchBristol Renal, Bristol Medical School, University of Bristol, Bristol, UK; Bristol Veterinary School, University of Bristol, Langford, UK; Langford Vets, Langford House, Langford, UK.

University of Bristol · GBRoyal Veterinary College · GBThe Langton Grammar School for Boys · GB

Funding

Wellcome Trust

6 · The paper itself

Abstract

The endothelial glycocalyx (eGlx) lines the luminal surface of endothelial cells. It is critical in maintaining vascular health and when damaged contributes to many diseases. Its fragility makes studying the eGlx technically challenging. The current reference standard for eGlx visualisation, by electron microscopy using glutaraldehyde/Alcian blue perfusion fixation, has not been previously reported in dogs. Established techniques were applied to achieve visualisation of the eGlx in the microvasculature of reproductive tissue in five healthy dogs undergoing elective neutering. Uterine and testicular artery samples underwent perfusion fixation, in the presence of Alcian blue, prior to transmission electron microscopy imaging. Image processing software was used to determine eGlx depth. EGlx was visualised in the arteries of two dogs, one testicular and one uterine, with median (range) eGlx depths of 68.2 nm (32.1-122.9 nm) and 47.6 nm (26.1-129.4 nm) respectively. Study of the eGlx is technically challenging, particularly its direct visualisation in clinical samples. Further research is needed to develop more clinically applicable techniques to measure eGlx health.

Indexed as

Endothelial CellsGlycocalyxAlcian BlueAnimalsDogsPerfusionAlcian BlueAlcian blueCanineMicrovasculatureTransmission electron microscopy

Identifiers

PMID35640795

DOI10.1016/j.tvjl.2022.105844

PMCPMC9587350

OpenAlexW4281736130

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.