Evidence map›Paper›PMID 35660507›Full record

ArticleAnalytical biochemistry2022

Selective isolation of hyaluronan by solid phase adsorption to silica.

Rebecca MacLeod, Fok Vun Chan, Han Yuan, Xin Ye, Yun Jin Ashley Sin, Teraesa M Vitelli, Tudor Cucu, Annie Leung, Irene Baljak, Samantha Osinski and 6 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 60% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 2 countries.

Rebecca MacLeodDepartment of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, NY, 10010, USA. Electronic address: rmacleod@maceps.com.
Fok Vun ChanEchelon Biosciences Inc., 675 Arapeen Drive, Suite 302, Salt Lake City, UT, 84108, USA. Electronic address: schan@echelon-inc.com.
Han YuanDepartment of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: julianypoly@gmail.com.
Xin YeDepartment of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: yexin2011@gmail.com.
Yun Jin Ashley SinDepartment of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, NY, 10010, USA. Electronic address: yjs287@nyu.edu.
Teraesa M VitelliDepartment of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, NY, 10010, USA. Electronic address: tmv7271@nyu.edu.
Tudor CucuDepartment of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: coross2@gmail.com.
Annie LeungDepartment of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: al4631@nyu.edu.
Irene BaljakDepartment of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, NY, 10010, USA. Electronic address: irene96b@gmail.com.
Samantha OsinskiDepartment of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: samanthaosinski@gmail.com.
Yuhong FuDepartment of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: fyhong313@outlook.com.
Gyu Ik Daniel JungDepartment of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: gj547@nyu.edu.
Anant AmarDepartment of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: anant.amar@my.rfums.org.
Paul L DeAngelisDepartment of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, 940 Stanton L. Young Blvd., Oklahoma, OK, 73104, USA. Electronic address: paul-deangelis@ouhsc.edu.
Urban HellmanDepartment of Public Health and Clinical Medicine, Umeå University, SE-901 87, Umeå, Sweden. Electronic address: urban.hellman@umu.se.
Mary K CowmanDepartment of Biomedical Engineering, Tandon School of Engineering, New York University, 433 First Avenue, 9thfloor, New York, NY, 10010, USA; Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, New York University, 6 Metrotech Center, Brooklyn, NY, 11201, USA. Electronic address: mary.cowman@nyu.edu.
New York University · USEchelon Biosciences · USUmeå University · SEUniversity of Oklahoma Health Sciences Center · US

Funding

Accessible And Robust Tools for Glycosaminoglycan ProfilingR43GM131444 · NIGMS · ECHELON BIOSCIENCES, INC. · PI CHAN, FOK VUN · 2019 to 2020
$300k
NIGMS NIH HHS R43 GM131444
6 · The paper itself

Abstract

A solid phase adsorption method for selective isolation of hyaluronan (HA) from biological samples is presented. Following enzymatic degradation of protein, HA can be separated from sulfated glycosaminoglycans, other unsulfated glycosaminoglycans, nucleic acids, and proteolytic fragments by adsorption to amorphous silica at specific salt concentrations. The adsorbed HA can be released from silica using neutral and basic aqueous solutions. HA ranging in size from ∼9 kDa to MDa polymers has been purified by this method from human serum and conditioned medium of cultured cells.

Indexed as

Hyaluronic AcidSilicon DioxideAdsorptionCells, CulturedGlycosaminoglycansHumansGlycosaminoglycansHyaluronic AcidSilicon DioxideConditioned mediumGlycosaminoglycanHyaluronanIsolationSerumSilica

Identifiers

PMID35660507
PMCPMC9589902
OpenAlexW4281732071

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.