Evidence map›Paper›PMID 35673908›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2022

[Interference of P2X4 receptor expression in tumor-associated macrophages suppresses migration and invasion of glioma cells].

X Yang, H Shen, Q Li, Z Dai, R Yang, G Huang, R Chen, F Wang, J Song, H Hua

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

X YangDepartment of Pathology and Pathophysiology, Kunming Medical University, Kunming 650500, China.
H ShenDepartment of Pathology, Zhaotong First People's Hospital, Zhaotong 657099, China.
Q LiClinic Skill Center, Kunming Medical University, Kunming 650500, China.
Z DaiInstitute of Stomatology, Affiliated Stomatology Hospital, Kunming Medical University, Kunming 650500, China.
R YangInstitute of Stomatology, Affiliated Stomatology Hospital, Kunming Medical University, Kunming 650500, China.
G HuangInstitute of Stomatology, Affiliated Stomatology Hospital, Kunming Medical University, Kunming 650500, China.
R ChenInstitute of Stomatology, Affiliated Stomatology Hospital, Kunming Medical University, Kunming 650500, China.
F WangDepartment of Pathology and Pathophysiology, Kunming Medical University, Kunming 650500, China.
J SongElectron Microscope, Kunming Medical University, Kunming 650500, China.
H HuaDepartment of Pathology and Pathophysiology, Kunming Medical University, Kunming 650500, China.
Kunming Medical University · CNKunming University · CNThe First People's Hospital of Zhaoqing · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the effect of interference of P2X4 receptor expression in tumor-associated macrophages (TAMs) on invasion and migration of glioma cells.

methodsC57BL/6 mouse models bearing gliomas in the caudate nucleus were examined for glioma pathology with HE staining and expressions of Iba-1 and P2X4 receptor with immunofluorescence assay. RAW264.7 cells were induced into TAMs using conditioned medium from GL261 cells, and the changes in mRNA expressions of macrophage polarization-related markers and the mRNA and protein expressions of P2X4 receptor were detected with RT-qPCR and Western blotting. The effect of siRNA-mediated P2X4 interference on IL-1β and IL-18 mRNA and protein expressions in the TAMs was detected with RT-qPCR and Western blotting. GL261 cells were cultured in the conditioned medium from the transfected TAMs, and the invasion and migration abilities of the cells were assessed with Transwell invasion and migration experiment.

resultsThe glioma tissues from the tumor-bearing mice showed a significantly greater number of Iba-1-positive cells, where an obviously increased P2X4 receptor expression was detected (

conclusionInterference of P2X4 receptor in the TAMs suppresses the migration and invasion of glioma cells possibly by lowering the expressions of IL-1β and IL-18.

Indexed as

GliomaTumor-Associated MacrophagesAnimalsCulture Media, ConditionedInterleukin-18MiceMice, Inbred C57BLReceptors, Purinergic P2X4RNA, MessengerCulture Media, ConditionedInterleukin-18P2rx4 protein, mouseReceptors, Purinergic P2X4RNA, MessengergliomainvasionmigrationP2X4 receptortumor associated macrophages

Identifiers

PMID35673908
PMCPMC9178632
OpenAlexW4282945943

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.