ArticleFoods (Basel, Switzerland)2022
Isolation and Characterization of Chicken Serum Albumin (Hen Egg Alpha-Livetin, Gal d 5).
Article in Foods (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Enhanced endocrine-metabolic support and axonemal assembly in high-sperm-motility geese: insights from testicular cellular heterogeneity by scRNA-seq.Poultry science · 2026Article
- Feed Components and Egg Allergenicity: Impact of Lupin and Soybean Meal Inclusion on Hen Egg Immunoreactivity (ELISA-Based Study).Foods (Basel, Switzerland) · 2026Article
- Novel food processing technologies to reduce egg allergenicity: a review from allergen digestion to IgE-mediated responses.Food science of animal resources · 2026Review
- Meat allergy: Specific reactions to chicken, beef, pork, and alternative protein sources.Asia Pacific allergy · 2026Review
- Metabolic and Endocrine Markers of Oregano Essential Oil Effects on Antibacterial Immunity, Reproductive Function, Nutritional Status, and Production Performance of Late-Phase Laying Hens.Veterinary sciences · 2025Article
- Insights into Isolation and Purification Strategies of Egg Allergens.Foods (Basel, Switzerland) · 2025Review
- A cross-species proteomic assessment of cost-effective platforms for depleting high-abundant proteins from blood serum.BMC methods · 2025Article
- Harnessing Natural Polymers for Nano-Scaffolds in Bone Tissue Engineering: A Comprehensive Overview of Bone Disease Treatment.Current issues in molecular biology · 2024Review
- The Impact of Processing and Extraction Methods on the Allergenicity of Targeted Protein Quantification as Well as Bioactive Peptides Derived from Egg.Molecules (Basel, Switzerland) · 2023Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Chicken serum albumin, i.e., hen egg alpha-livetin, is a recognized food allergen in chicken meat and hen eggs. Currently, there is no immunoassay available for its detection from food matrices. The characterization of chicken serum albumin-specific antibodies and the extraction of the target protein are essential for immunoassay development. One monoclonal antibody (mAb), 3H4, was used in this study due to its selectivity to a linear epitope on avian serum albumin. To study the extraction of chicken serum albumin, phosphate-buffered saline (PBS) with two additives, i.e., sodium dodecyl sulfate (SDS) and dithiothreitol (DTT), was used for its extraction from chicken blood plasma and hen egg yolk. SDS and DTT improved the chicken serum albumin's recovery and enhanced chicken serum albumin's immunodetection. In addition, chicken serum albumin retained the best solubility and immunoreactivity after heat treatment in a neutral condition. It experienced degradation and aggregation in acidic and alkaline conditions, respectively. Overall, PBS containing 0.1% SDS and 1 mM DTT (pH 7.2) was a better extraction buffer for chicken serum albumin. However, the complexity of the food matrix and elevated temperature could reduce its solubility and immunoreactivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.