ArticleMolecular systems biology2022
Optogenetic actuator - ERK biosensor circuits identify MAPK network nodes that shape ERK dynamics.
Article in Molecular systems biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 50 citations in OpenAlex.
- Article
- Quantifying cancer- and drug-induced changes in Shannon information capacity of RTK signaling.Scientific reports · 2025Article
- Methods for Controlling Small GTPase Activity.Chembiochem : a European journal of chemical biology · 2025Review
- Mathematical Modeling and Inference of Epidermal Growth Factor-Induced Mitogen-Activated Protein Kinase Cell Signaling Pathways.International journal of molecular sciences · 2024Review
- Oncogenic Kras induces spatiotemporally specific tissue deformation through converting pulsatile into sustained ERK activation.Nature cell biology · 2024Article
- Deep model predictive control of gene expression in thousands of single cells.Nature communications · 2024Article
- Optogenetic Regulation of EphA1 RTK Activation and Signaling.bioRxiv : the preprint server for biology · 2024Article
- Rapid Optogenetic Clustering in the Cytoplasm with BcLOVclust.Journal of molecular biology · 2024Article
- Light-inducible protein degradation ineLife · 2024Article
- A guide to ERK dynamics, part 1: mechanisms and models.The Biochemical journal · 2023Article
- An improved Erk biosensor detects oscillatory Erk dynamics driven by mitotic erasure during early development.Developmental cell · 2023Article
- Automatic detection of spatio-temporal signaling patterns in cell collectives.The Journal of cell biology · 2023Article
- Rapid optogenetic clustering of a cytoplasmic BcLOV4 variant.bioRxiv : the preprint server for biology · 2023Article
- The MAPK/ERK channel capacity exceeds 6 bit/hour.PLoS computational biology · 2023Article
- Dynamic regulation of RAS and RAS signaling.The Biochemical journal · 2023Article
- LITOS: a versatile LED illumination tool for optogenetic stimulation.Scientific reports · 2022Article
- Optogenetic activators of apoptosis, necroptosis, and pyroptosis.The Journal of cell biology · 2022Article
- Optogenetic actuator - ERK biosensor circuits identify MAPK network nodes that shape ERK dynamics.Molecular systems biology · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Combining single-cell measurements of ERK activity dynamics with perturbations provides insights into the MAPK network topology. We built circuits consisting of an optogenetic actuator to activate MAPK signaling and an ERK biosensor to measure single-cell ERK dynamics. This allowed us to conduct RNAi screens to investigate the role of 50 MAPK proteins in ERK dynamics. We found that the MAPK network is robust against most node perturbations. We observed that the ERK-RAF and the ERK-RSK2-SOS negative feedback operate simultaneously to regulate ERK dynamics. Bypassing the RSK2-mediated feedback, either by direct optogenetic activation of RAS, or by RSK2 perturbation, sensitized ERK dynamics to further perturbations. Similarly, targeting this feedback in a human ErbB2-dependent oncogenic signaling model increased the efficiency of a MEK inhibitor. The RSK2-mediated feedback is thus important for the ability of the MAPK network to produce consistent ERK outputs, and its perturbation can enhance the efficiency of MAPK inhibitors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.