ArticleNature cell biology2022
Integrated multi-omics reveal polycomb repressive complex 2 restricts human trophoblast induction.
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Article in Nature cell biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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0cells of the map it votes in
41citing papers in PubMed
6.1field-weighted citation impact, top 2% of its field
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 72 citations in OpenAlex.
- An Integrated Proteomics and Genomics Approach to Identify Essential Protein Kinases During Human Trophoblast Development.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Reversible epiblast regionalization determines differentiation potential of human pluripotent stem cells.Nature biotechnology · 2026Article
- Opposing function of AEBP2 isoforms fine-tune PRC2 catalytic activity.Nucleic acids research · 2026Article
- Multiple strategies to resolve the conflict between replication and transcription in mammalian cells.Nucleic acids research · 2026Article
- Exiting the ground state: the broad spectrum of cell fates accessible from naïve human pluripotent stem cells.Cellular and molecular life sciences : CMLS · 2026Review
- Placental biology links genetic, epigenetic, ancestral, and social determinants to maternal-fetal health inequities.Frontiers in reproductive health · 2026Review
- METTL3 safeguards cell identity and epigenome of human trophoblast stem cells.Nature communications · 2025Article
- Double stranded RNA sensing is silenced during early embryonic development.Nature communications · 2025Article
- VGLL1 contributes to both the transcriptome and epigenome of the developing trophoblast compartment.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A conserved function of EZH2-PRC2 in repression of primitive endoderm fate in mouse and human pluripotent cells.Stem cell reports · 2025Article
- Review
- Aflatoxin B1 induces subtle but coordinated histone modifications in Epstein-Barr virus infected and non-infected Burkitt lymphoma cells.Environment international · 2025Article
- Establishment of human expanded potential stem cell lines via preimplantation embryo cultivation and somatic cell reprogramming.Nature protocols · 2025Review
- D-Mannose-Mediated metabolic pathways sustain the molecular signatures of sperm function and fertilization.Journal of advanced research · 2025Article
- Polycomb repressive complexes 1 and 2 independently and dynamically regulate euchromatin during cerebellar neurodevelopment.PLoS genetics · 2025Article
- Dynamic Proteome Landscape During Preimplantation Human Embryo Development and Trophectoderm Stem Cell-Differentiation.Proteomics · 2025Article
- α-Ketoglutarate promotes trophectoderm induction and maturation from naive human embryonic stem cells.Nature cell biology · 2025Article
- Inhibition of PRC2 enables self-renewal of blastoid-competent naive pluripotent stem cells from chimpanzee.Cell stem cell · 2025Article
- Transient chemical-mediated epigenetic modulation confers unrestricted lineage potential on human primed pluripotent stem cells.Science China. Life sciences · 2025Article
- H3K27 dimethylation dynamics reveal stepwise establishment of facultative heterochromatin in early mouse embryos.Nature cell biology · 2025Article
Corrections and comments
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Authors and funding
37 authors at 8 institutions in 5 countries.
Dick W Zijlmans *Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences (RIMLS), Oncode Institute, Radboud University Nijmegen, Nijmegen, The Netherlands.
Irene Talon *Department of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0002-5800-5555
Sigrid Verhelst *ProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University, Ghent, Belgium.ORCID http://orcid.org/0000-0002-6791-3782
Adam Bendall *Epigenetics Programme, Babraham Institute, Cambridge, UK.ORCID http://orcid.org/0000-0002-6865-2625
Karlien Van NerumDepartment of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0002-8952-5473
Alok JavaliInstitute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.
Andrew A MalcolmEpigenetics Programme, Babraham Institute, Cambridge, UK.ORCID http://orcid.org/0000-0001-6240-7701
Sam S F A van KnippenbergDepartment of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0001-5656-3046
Laura BigginsBioinformatics Group, Babraham Institute, Cambridge, UK.
San Kit ToDepartment of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0002-3057-2902
Adrian JaniszewskiDepartment of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.
Danielle AdmiraalDepartment of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences (RIMLS), Radboud University Nijmegen, Nijmegen, The Netherlands.
Ruth KnopsLaboratory of Hematology, Department of Laboratory Medicine, Radboud University Nijmegen Medical Centre (RadboudUMC), Nijmegen, the Netherlands.
Nikky CorthoutVIB Center for Brain and Disease Research, KU Leuven, VIB Bioimaging Core, Leuven, Belgium.
Bradley P BalatonDepartment of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0002-5130-7868
Grigorios GeorgolopoulosDepartment of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.
Amitesh PandaDepartment of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0002-2702-3494
Natarajan V BhanuEpigenetics Institute, Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Amanda J CollierEpigenetics Programme, Babraham Institute, Cambridge, UK.
Charlene FabianEpigenetics Programme, Babraham Institute, Cambridge, UK.
Ryan N AllsopDepartment of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.
Joel ChappellDepartment of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.
Thi Xuan Ai PhamDepartment of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0003-3866-2002
Michael OberhuemerDepartment of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0003-2273-2782
Cankat ErtekinDepartment of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.
Lotte VanheerDepartment of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.
Paraskevi AthanasouliDepartment of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.
Frederic LluisDepartment of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium.
Dieter DeforceProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University, Ghent, Belgium.ORCID http://orcid.org/0000-0002-0635-661X
Joop H JansenLaboratory of Hematology, Department of Laboratory Medicine, Radboud University Nijmegen Medical Centre (RadboudUMC), Nijmegen, the Netherlands.
Benjamin A GarciaEpigenetics Institute, Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Michiel VermeulenDepartment of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences (RIMLS), Oncode Institute, Radboud University Nijmegen, Nijmegen, The Netherlands.ORCID http://orcid.org/0000-0003-0836-6894
Nicolas RivronInstitute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.ORCID http://orcid.org/0000-0003-1590-5964
Maarten DhaenensProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University, Ghent, Belgium. Maarten.Dhaenens@UGent.be.ORCID http://orcid.org/0000-0002-9801-3509
Hendrik MarksDepartment of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences (RIMLS), Radboud University Nijmegen, Nijmegen, The Netherlands. Hendrik.Marks@ru.nl.ORCID http://orcid.org/0000-0002-4198-3731
Peter J Rugg-GunnEpigenetics Programme, Babraham Institute, Cambridge, UK. peter.rugg-gunn@babraham.ac.uk.ORCID http://orcid.org/0000-0002-9601-5949
Vincent PasqueDepartment of Development and Regeneration, Leuven Stem Cell Institute, Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven, Belgium. vincent.pasque@kuleuven.be.ORCID http://orcid.org/0000-0002-5129-0146
KU Leuven · BEBabraham Institute · GBRadboud University Nijmegen · NLGhent University · BEInstitute of Molecular Biotechnology · ATUniversity of Pennsylvania · USVIB-KU Leuven Center for Brain & Disease Research · BEVIB-KU Leuven Center for Microbiology · BE
Funding
STRUCTURAL BASIS FOR EPIGENETICS IN AGING AND LONGEVITYP01AG031862 · NIA · WISTAR INSTITUTE · PI ZHANG, RUGANG · 2008 to 2022
$28.3MShared Resources Core 2: Quantitative Proteomics CoreP01CA196539 · NCI · ROCKEFELLER UNIVERSITY · PI YOUNG, MICHAEL WARREN · 2015 to 2024
$17.6MViral modulation of epitranscriptomic mechanismsR01AI118891 · NIAID · WASHINGTON UNIVERSITY · PI GARCIA, BENJAMIN A, WEITZMAN, MATTHEW D. · 2015 to 2025
$5.4MBiotechnology and Biological Sciences Research Council BBS/E/B/000C0421Biotechnology and Biological Sciences Research Council BBS/E/B/000C0422Medical Research Council MR/J003808/1Medical Research Council MR/N018419/1Medical Research Council MR/T011769/1Medical Research Council MR/V02969X/1NCI NIH HHS P01 CA196539NIAID NIH HHS R01 AI118891NIA NIH HHS P01 AG031862Wellcome TrustWellcome Trust 102160/Z/13/ZWellcome Trust 215116/Z/18/Z
Abstract
Human naive pluripotent stem cells have unrestricted lineage potential. Underpinning this property, naive cells are thought to lack chromatin-based lineage barriers. However, this assumption has not been tested. Here we define the chromatin-associated proteome, histone post-translational modifications and transcriptome of human naive and primed pluripotent stem cells. Our integrated analysis reveals differences in the relative abundance and activities of distinct chromatin modules. We identify a strong enrichment of polycomb repressive complex 2 (PRC2)-associated H3K27me3 in the chromatin of naive pluripotent stem cells and H3K27me3 enrichment at promoters of lineage-determining genes, including trophoblast regulators. PRC2 activity acts as a chromatin barrier restricting the differentiation of naive cells towards the trophoblast lineage, whereas inhibition of PRC2 promotes trophoblast-fate induction and cavity formation in human blastoids. Together, our results establish that human naive pluripotent stem cells are not epigenetically unrestricted, but instead possess chromatin mechanisms that oppose the induction of alternative cell fates.
Indexed as
Pluripotent Stem CellsPolycomb Repressive Complex 2Cell DifferentiationChromatinHistonesHumansTrophoblastsChromatinHistonesPolycomb Repressive Complex 2
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