Evidence map›Paper›PMID 35700317›Full record

ArticleJournal of the American Chemical Society2022

Selective Isotope Labeling and LC-Photo-CIDNP Enable NMR Spectroscopy at Low-Nanomolar Concentration.

Hanming Yang, Siyu Li, Clayton A Mickles, Valeria Guzman-Luna, Kenji Sugisaki, Clayton M Thompson, Hung H Dang, Silvia Cavagnero

Erratum issuedOpen access · greenAbstract read
In one paragraph

Article in Journal of the American Chemical Society, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
3.9field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 36 citations in OpenAlex.

  1. Review
  2. Fluorinated Tryptophan Derivatives for Photo-CIDNP NMR.The journal of physical chemistry. B · 2026
    Article
  3. Nuclear-Spin Hyperpolarization of Biomolecules by Optically Enhanced NMR at Ultra-High Field.Journal of the Chinese Chemical Society. Zhongguo hua xue hui (Taipei, Taiwan) · 2026
    Article
  4. Applications of quantitativeJournal of pharmaceutical analysis · 2026
    Review
  5. Continuous-Wave (CW) Photo-CIDNP NMR Spectroscopy: A Tutorial.Magnetic resonance in chemistry : MRC · 2025
    Article
  6. Article
  7. Mechanism of Solid-StateThe journal of physical chemistry letters · 2024
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Ultrafast Fragment Screening Using Photo-Hyperpolarized (CIDNP) NMR.Journal of the American Chemical Society · 2023
    Article
  14. Review
  15. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Hanming YangDepartment of Chemistry, University of Wisconsin─Madison, 1101 University Avenue, Madison, Wisconsin 53706, United States.
Siyu LiDepartment of Chemistry, University of Wisconsin─Madison, 1101 University Avenue, Madison, Wisconsin 53706, United States.
Clayton A MicklesDepartment of Chemistry, University of Wisconsin─Madison, 1101 University Avenue, Madison, Wisconsin 53706, United States.
Valeria Guzman-LunaDepartment of Chemistry, University of Wisconsin─Madison, 1101 University Avenue, Madison, Wisconsin 53706, United States.
Kenji SugisakiDepartment of Chemistry, Graduate School of Science, Osaka Metropolitan University, 3-3-138 Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan.ORCID 0000-0002-1950-5725
Clayton M ThompsonDepartment of Chemistry, University of Wisconsin─Madison, 1101 University Avenue, Madison, Wisconsin 53706, United States.
Hung H DangDepartment of Chemistry, University of Wisconsin─Madison, 1101 University Avenue, Madison, Wisconsin 53706, United States.
Silvia CavagneroDepartment of Chemistry, University of Wisconsin─Madison, 1101 University Avenue, Madison, Wisconsin 53706, United States.ORCID 0000-0002-4290-2331
University of Wisconsin–Madison · USJapan Science and Technology Agency · JP

Funding

Development of LED-Assisted NMR Technologies for the Atomic-Resolution Analysis of Medically Relevant Biomolecules in Solution at Submicromolar ConcentrationR01GM125995 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI CAVAGNERO, SILVIA · 2018 to 2025
$2.8M
Laser-Enhanced NMR Instrumentation for Chemical and Biochemical ResearchS10OD012245 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI CAVAGNERO, SILVIA · 2013 to 2013
$1.0M
NIGMS NIH HHS R01 GM125995NIH HHS S10 OD012245
6 · The paper itself

Abstract

NMR spectroscopy is a powerful tool to investigate molecular structure and dynamics. The poor sensitivity of this technique, however, limits its ability to tackle questions requiring dilute samples. Low-concentration photochemically induced dynamic nuclear polarization (LC-photo-CIDNP) is an optically enhanced NMR technology capable of addressing the above challenge by increasing the detection limit of aromatic amino acids in solution up to 1000-fold, either in isolation or within proteins. Here, we show that the absence of NMR-active nuclei close to a magnetically active site of interest (e.g., the structurally diagnostic

Indexed as

ProteinsTryptophanAmino AcidsIsotope LabelingMagnetic Resonance SpectroscopyAmino AcidsProteinsTryptophan

Identifiers

PMID35700317
PMCPMC9577358
OpenAlexW4282827940

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.