ArticleThe EMBO journal2022
Nucleome programming is required for the foundation of totipotency in mammalian germline development.
Article in The EMBO journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- ATAC-seq of low-input and cryopreserved primordial germ cells reveals functional enhancers.Development (Cambridge, England) · 2026Article
- Reprogramming the inactive X chromosome: dynamics and insights from the germline.Biochemical Society transactions · 2026Review
- Global reorganization of genome architecture at the transition to gametogenesis.Nature structural & molecular biology · 2026Article
- The 3D genome during germline development and meiosis.Trends in genetics : TIG · 2026Review
- Mechanisms of human germ cell development.Nature reviews. Molecular cell biology · 2026Review
- The mitotic STAG3-cohesin complex shapes male germline nucleome.Nature structural & molecular biology · 2025Article
- CTCF-mediated 3D chromatin sets up the gene expression program in the male germline.Nature structural & molecular biology · 2025Article
- Article
- Epigenetic priming in the male germline.Current opinion in genetics & development · 2024Review
- A comprehensive benchmarking with interpretation and operational guidance for the hierarchy of topologically associating domains.Nature communications · 2024Article
- Reinforcement of repressive marks in the chicken primordial germ cell epigenetic signature: divergence from basal state resetting in mammals.Epigenetics & chromatin · 2024Article
- MAX controls meiotic entry in sexually undifferentiated germ cells.Scientific reports · 2024Article
- Cohesin and CTCF do not assemble TADs inGenome research · 2023Article
- Stage-specific dynamic reorganization of genome topology shapes transcriptional neighborhoods in developing human retinal organoids.Cell reports · 2023Article
- Recent Progress of In Vitro 3D Culture of Male Germ Stem Cells.Journal of functional biomaterials · 2023Review
- Evidence for low nanocompaction of heterochromatin in living embryonic stem cells.The EMBO journal · 2023Article
- Transgenerational inheritance and its modulation by environmental cues.Current topics in developmental biology · 2023Article
- Nucleome programming is required for the foundation of totipotency in mammalian germline development.The EMBO journal · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
30 authors at 8 institutions in 5 countries.
Funding
Abstract
Germ cells are unique in engendering totipotency, yet the mechanisms underlying this capacity remain elusive. Here, we perform comprehensive and in-depth nucleome analysis of mouse germ-cell development in vitro, encompassing pluripotent precursors, primordial germ cells (PGCs) before and after epigenetic reprogramming, and spermatogonia/spermatogonial stem cells (SSCs). Although epigenetic reprogramming, including genome-wide DNA de-methylation, creates broadly open chromatin with abundant enhancer-like signatures, the augmented chromatin insulation safeguards transcriptional fidelity. These insulatory constraints are then erased en masse for spermatogonial development. Notably, despite distinguishing epigenetic programming, including global DNA re-methylation, the PGCs-to-spermatogonia/SSCs development entails further euchromatization. This accompanies substantial erasure of lamina-associated domains, generating spermatogonia/SSCs with a minimal peripheral attachment of chromatin except for pericentromeres-an architecture conserved in primates. Accordingly, faulty nucleome maturation, including persistent insulation and improper euchromatization, leads to impaired spermatogenic potential. Given that PGCs after epigenetic reprogramming serve as oogenic progenitors as well, our findings elucidate a principle for the nucleome programming that creates gametogenic progenitors in both sexes, defining a basis for nuclear totipotency.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.