Evidence map›Paper›PMID 35711315›Full record

ArticleFrontiers in physiology2022

Optimization of Whole Mount RNA Multiplexed

Ali M Elagoz, Ruth Styfhals, Sofia Maccuro, Luca Masin, Lieve Moons, Eve Seuntjens

Open access · goldAbstract read
In one paragraph

Article in Frontiers in physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
12.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 34 citations in OpenAlex.

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  12. Experimental Neurogenesis in the Embryos of the Gecko Paroedura picta.Methods in molecular biology (Clifton, N.J.) · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Ali M ElagozLaboratory of Developmental Neurobiology, Department of Biology, KU Leuven, Leuven, Belgium.
Ruth StyfhalsLaboratory of Developmental Neurobiology, Department of Biology, KU Leuven, Leuven, Belgium.
Sofia MaccuroLaboratory of Developmental Neurobiology, Department of Biology, KU Leuven, Leuven, Belgium.
Luca MasinLeuven Brain Institute, KU Leuven, Leuven, Belgium.
Lieve MoonsLeuven Brain Institute, KU Leuven, Leuven, Belgium.
Eve SeuntjensLaboratory of Developmental Neurobiology, Department of Biology, KU Leuven, Leuven, Belgium.
VIB-KU Leuven Center for Brain & Disease Research · BEKU Leuven · BEStazione Zoologica Anton Dohrn · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gene expression analysis has been instrumental to understand the function of key factors during embryonic development of many species. Marker analysis is also used as a tool to investigate organ functioning and disease progression. As these processes happen in three dimensions, the development of technologies that enable detection of gene expression in the whole organ or embryo is essential. Here, we describe an optimized protocol of whole mount multiplexed RNA

Indexed as

brain developmentcephalopodfluorescent in situ hybridizationimmunohistochemistryinvertebratelight sheet imagingtissue clearing

Identifiers

PMID35711315
PMCPMC9196907
OpenAlexW4281690499

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.