Evidence map›Paper›PMID 35733606›Full record

ArticleSTAR protocols2022

Protocol for live cell image segmentation to profile cellular morphodynamics using MARS-Net.

Junbong Jang, Caleb Hallinan, Kwonmoo Lee

Abstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Unsupervised Contour Tracking of Live Cells by Mechanical and Cycle Consistency Losses.Proceedings. IEEE Computer Society Conference on Computer Vision and Pattern Recognition · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Junbong JangVascular Biology Program, Boston Children's Hospital, Boston, MA 02115, USA. Electronic address: junbongjang@kaist.ac.kr.
Caleb HallinanVascular Biology Program, Boston Children's Hospital, Boston, MA 02115, USA.
Kwonmoo LeeVascular Biology Program, Boston Children's Hospital, Boston, MA 02115, USA; Department of Surgery, Harvard Medical School, Boston, MA 02115, USA. Electronic address: kwonmoo.lee@childrens.harvard.edu.

Funding

Unraveling subcellular heterogeneity of molecular coordination by machine learningR35GM133725 · NIGMS · WORCESTER POLYTECHNIC INSTITUTE · PI Kwonmoo Lee · 2019 to 2026
$3.9M
Spatiotemporal Coordination of Actin Regulators and Traction Forces in Heterogeneous Cell ProtrusionsR15GM122012 · NIGMS · WORCESTER POLYTECHNIC INSTITUTE · PI LEE, KWONMOO · 2017 to 2017
$450k
NIGMS NIH HHS R15 GM122012NIGMS NIH HHS R35 GM133725
6 · The paper itself

Abstract

Quantitative studies of cellular morphodynamics rely on accurate cell segmentation in live cell images. However, fluorescence and phase contrast imaging hinder accurate edge localization. To address this challenge, we developed MARS-Net, a deep learning model integrating ImageNet-pretrained VGG19 encoder and U-Net decoder trained on the datasets from multiple types of microscopy images. Here, we provide the protocol for installing MARS-Net, labeling images, training MARS-Net for edge localization, evaluating the trained models' performance, and performing the quantitative profiling of cellular morphodynamics. For complete details on the use and execution of this protocol, please refer to Jang et al. (2021).

Indexed as

Image Processing, Computer-AssistedNeural Networks, ComputerBioinformaticsCell BiologyComputer sciencesMicroscopy

Identifiers

PMID35733606
PMCPMC9207580

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.