Evidence map›Paper›PMID 35756686›Full record

ArticleFrontiers in oncology2022

Successful Preservation of Native BCR::ABL1 in Chronic Myeloid Leukemia Primary Leukocytes Reveals a Reduced Kinase Activity.

Christian Boni, Massimiliano Bonifacio, Marzia Vezzalini, Luigi Scaffidi, Luisa Tomasello, Laurie L Parker, Diego Boscarino, Dino Paladin, Mauro Krampera, Claudio Sorio

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in oncology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 86% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Christian BoniDepartment of Medicine, General Pathology Section, University of Verona, Verona, Italy.
Massimiliano BonifacioDepartment of Medicine, Hematology Section, University of Verona, Verona, Italy.
Marzia VezzaliniDepartment of Medicine, General Pathology Section, University of Verona, Verona, Italy.
Luigi ScaffidiDepartment of Medicine, Hematology Section, University of Verona, Verona, Italy.
Luisa TomaselloDepartment of Medicine, General Pathology Section, University of Verona, Verona, Italy.
Laurie L ParkerDepartment of Biochemistry, Molecular Biology and Biophysics, Masonic Cancer Center, University of Minnesota, Minneapolis, United States.
Diego BoscarinoAB Analitica, Padova, Italy.
Dino PaladinAB Analitica, Padova, Italy.
Mauro KramperaDepartment of Medicine, Hematology Section, University of Verona, Verona, Italy.
Claudio SorioDepartment of Medicine, General Pathology Section, University of Verona, Verona, Italy.
University of Verona · ITAb Analitica (Italy) · ITUniversity of Minnesota · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chronic myeloid leukemia (CML) is a myeloproliferative disease caused by the acquisition of t(9;22) generating the fusion tyrosine kinase BCR::ABL1. However, despite the crucial role of this protein in the dysregulation of numerous signal transduction pathways, a direct measure of BCR::ABL1 kinase activity in chronic phase (CP) CML was never accomplished due to intense degradative activity present in mature leukocytes. Therefore, we developed a procedure suitable to preserve BCR::ABL1 protein under non-denaturing, neutral pH conditions in primary, chronic phase (CP)-CML samples. As a result, specific kinase activity was detected utilizing a biotinylated peptide substrate highly selective for c-ABL1. Furthermore, through this approach, BCR::ABL1 kinase activity was barely detectable in CP-CML compared to Ph

Indexed as

acute lymphocytic leukemiaBcr Ablchronic myelogenous leukemiaimatinib (Gleevec)kinase activitykinase assayphiladelphia chromosometyrosine kinase

Identifiers

PMID35756686
PMCPMC9216732
OpenAlexW4285744847

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.