Evidence map›Paper›PMID 35766215›Full record

ArticleJournal of histotechnology2022

A new method for the sampling and preservation of placental specimens in low-resource settings for the identification of

Patrick S Potoczak, Beverly I Strassmann, Claudius Vincenz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of histotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Patrick S PotoczakDepartment of Anthropology, University of Michigan, Ann Arbor, MI, USA.ORCID 0000-0003-3324-0417
Beverly I StrassmannDepartment of Anthropology, University of Michigan, Ann Arbor, MI, USA.ORCID 0000-0002-3783-7977
Claudius VincenzResearch Center for Group Dynamics, Institute for Social Research, University of Michigan, Ann Arbor, MI, USA.ORCID 0000-0002-2199-3609

Funding

Effect of the Placental Epigenome on Stunting in a Longitudinal African CohortR01HD088521 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI STRASSMANN, BEVERLY ILSE · 2016 to 2020
$3.0M
A longitudinal study of stunting and growth modulating genes in human placentasR21HD077465 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI STRASSMANN, BEVERLY ILSE · 2014 to 2015
$421k
NICHD NIH HHS R01 HD088521NICHD NIH HHS R21 HD077465
6 · The paper itself

Abstract

Collection, preservation, and shipment of histological specimens in low-resource settings is challenging. We present a novel method that achieved excellent preservation of placental specimens from rural Mali by using formalin fixation, ethanol dehydration, and long-term storage in a solar-powered freezer. Sample preservation success was 92%, permitting evaluation of current and past malaria infection, anemia, placental maturity, and inflammation. Using RNAscope® hybridization we were able to visualize cell-specific gene expression patterns in the formalin-fixed paraffin-embedded (FFPE) specimens. Additionally, our method entailed mirrored sampling from the two cut faces of a cotyledon, one for the FFPE workflows and the other for storage in RNAlater™ and RNA-seq.

Indexed as

Nucleic AcidsFemaleFormaldehydeHumansParaffin EmbeddingPlacentaPregnancyTissue FixationFormaldehydeNucleic AcidsField-workhistologymalariaplacentaRNAscope®RNA-seq

Identifiers

PMID35766215
PMCPMC9437128

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.