ArticleBiomedical optics express2022
Correlative imaging of the spatio-angular dynamics of biological systems with multimodal instant polarization microscope.
Article in Biomedical optics express, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Mantis: High-throughput 4D imaging and analysis of the molecular and physical architecture of cells.PNAS nexus · 2024Article
- Permittivity tensor imaging: modular label-free imaging of 3D dry mass and 3D orientation at high resolution.Nature methods · 2024Article
- Mantis: high-throughput 4D imaging and analysis of the molecular and physical architecture of cells.bioRxiv : the preprint server for biology · 2024Article
- Harnessing the power of optical microscopy for visualization and analysis of histopathological images.Biomedical optics express · 2023Review
- Polarization-sensitive intensity diffraction tomography.Light, science & applications · 2023Article
- High-fidelity optical diffraction tomography of live organisms using iodixanol refractive index matching.Biomedical optics express · 2022Article
- Correlative imaging of the spatio-angular dynamics of biological systems with multimodal instant polarization microscope.Biomedical optics express · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
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Abstract
The spatial and angular organization of biological macromolecules is a key determinant, as well as informative readout, of their function. Correlative imaging of the dynamic spatio-angular architecture of cells and organelles is valuable, but remains challenging with current methods. Correlative imaging of spatio-angular dynamics requires fast polarization-, depth-, and wavelength-diverse measurement of intrinsic optical properties and fluorescent labels. We report a multimodal instant polarization microscope (miPolScope) that combines a broadband polarization-resolved detector, automation, and reconstruction algorithms to enable label-free imaging of phase, retardance, and orientation, multiplexed with fluorescence imaging of concentration, anisotropy, and orientation of molecules at diffraction-limited resolution and high speed. miPolScope enabled multimodal imaging of myofibril architecture and contractile activity of beating cardiomyocytes, cell and organelle architecture of live HEK293T and U2OS cells, and density and anisotropy of white and grey matter of mouse brain tissue across the visible spectrum. We anticipate these developments in joint quantitative imaging of density and anisotropy to enable new studies in tissue pathology, mechanobiology, and imaging-based screens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.