Evidence map›Paper›PMID 35776324›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Immunofluorescence Staining of P2X7 Receptors in Whole-Mount Myenteric Plexus Preparations.

Tina Jooss, Birgit Kuch, Jiong Zhang, Michael Schemann, Annette Nicke

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Imaging the enteric nervous system.Frontiers in neuroanatomy · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Tina JoossWalther Straub Institute of Pharmacology and Toxicology, Faculty of Medicine, LMU Munich, Munich, Germany.
Birgit KuchDepartment of Human Biology, Technical University Munich, Freising-Weihenstephan, Germany.
Jiong ZhangWalther Straub Institute of Pharmacology and Toxicology, Faculty of Medicine, LMU Munich, Munich, Germany.
Michael SchemannDepartment of Human Biology, Technical University Munich, Freising-Weihenstephan, Germany.
Annette NickeWalther Straub Institute of Pharmacology and Toxicology, Faculty of Medicine, LMU Munich, Munich, Germany. annette.nicke@lrz.uni-muenchen.de.ORCID 0000-0001-6798-505X
Weihenstephan-Triesdorf University of Applied Sciences · DEJohannes Gutenberg University Mainz · DELMU Klinikum · DELudwig-Maximilians-Universität München · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

P2X7 receptors play an important role in cytokine release and immune cell regulation. Their upregulation has been described in inflammatory and degenerative processes and P2X7 blockade or deletion has been shown to reduce tissue damage and severity of symptoms in animal models of inflammatory bowel disease (IBD). Several studies have found that P2X7 receptors are present on enteric neurons and glia and it was proposed that they mediate neuronal death during IBD. However, the cell type-specific localization of P2X7 receptors has been a matter of debate, since some antibodies have been found to be unspecific. Here we describe the preparation of whole-mount myenteric plexus from the colon of BAC transgenic P2X7-EGFP reporter mice and subsequent immunofluorescence staining of P2X7 receptors together with cell type-specific marker proteins.

Indexed as

Inflammatory Bowel DiseasesMyenteric PlexusAnimalsFluorescent Antibody TechniqueMiceMice, TransgenicReceptors, Purinergic P2X7Staining and LabelingReceptors, Purinergic P2X7BAC transgenic P2X7-EGFP mouseF4/80Glial fibrillary acidic proteinHu C/DImmunofluorescenceP2X7−/− mouseP2X7 receptor

Identifiers

PMID35776324
OpenAlexW4285210011

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.