ArticleNature communications2022
Spatial regulation of AMPK signaling revealed by a sensitive kinase activity reporter.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed, 73 citations in OpenAlex.
- Calorie restriction and exercise differentially regulate AMP-activated protein kinase across subcellular compartments in skeletal muscle from older male rats.Experimental gerontology · 2026Article
- TFEB Antagonizes Cardiac Hypertrophy and Failure by Enhancing Lysosomal Capacity and Mitochondrial Function.Circulation research · 2026Article
- Article
- SNF1/AMPK controls its own localization by phosphorylating its activating kinase Sak1.iScience · 2026Article
- A Constellation of Fluorescent Biosensors to Illuminate the Galaxy of Protein Kinases.Chembiochem : a European journal of chemical biology · 2026Review
- Illuminating compartmentalized AMPK signaling in single cells.The Biochemical journal · 2026Review
- A Far-Red FRET biosensor for AMPK enables multiplexed imaging of single-cell bioenergetic homeostasis.bioRxiv : the preprint server for biology · 2026Article
- Lactate-Induced Lipid Accumulation in Hepatocytes through GPR81 Activation (Diabetes Metab J 2026;50: 307-19).Diabetes & metabolism journal · 2026Article
- Lactate-Induced Lipid Accumulation in Hepatocytes through GPR81 Activation (Diabetes Metab J 2026;50:307-19).Diabetes & metabolism journal · 2026Article
- Energy stress activates AMPK to arrest mitochondria via phosphorylation of TRAK1.The Journal of cell biology · 2026Article
- Review
- Visualizing PINK1 Activity Dynamics in Single Cells with a Phase Separation-Based Kinase Activity Reporter.ACS sensors · 2026Article
- AMPK at the interface of nutrient sensing, metabolic flux and energy homeostasis.Nature metabolism · 2026Review
- CellSAM: a foundation model for cell segmentation.Nature methods · 2025Article
- Two novel red-FRET ERK bosensors in the 670-720 nm range.Journal of biological engineering · 2025Article
- Molecular recording of cellular protein kinase activity with chemical labeling.Nature chemical biology · 2025Article
- Systems modeling and uncertainty quantification of AMP-activated protein kinase signaling.NPJ systems biology and applications · 2025Article
- Decoding Molecular Network Dynamics in Cells: Advances in Multiplexed Live Imaging of Fluorescent Biosensors.Biosensors · 2025Review
- Dissecting metabolic regulation of behaviors and physiology during aging in Drosophila.Biogerontology · 2025Article
- Genetically encoded biosensors of metabolic function for the study of neurodegeneration, a review and perspective.Neurophotonics · 2025Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
AMP-activated protein kinase (AMPK) is a master regulator of cellular energetics which coordinates metabolism by phosphorylating a plethora of substrates throughout the cell. But how AMPK activity is regulated at different subcellular locations for precise spatiotemporal control over metabolism is unclear. Here we present a sensitive, single-fluorophore AMPK activity reporter (ExRai AMPKAR), which reveals distinct kinetic profiles of AMPK activity at the mitochondria, lysosome, and cytoplasm. Genetic deletion of the canonical upstream kinase liver kinase B1 (LKB1) results in slower AMPK activity at lysosomes but does not affect the response amplitude at lysosomes or mitochondria, in sharp contrast to the necessity of LKB1 for maximal cytoplasmic AMPK activity. We further identify a mechanism for AMPK activity in the nucleus, which results from cytoplasmic to nuclear shuttling of AMPK. Thus, ExRai AMPKAR enables illumination of the complex subcellular regulation of AMPK signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.