Evidence map›Paper›PMID 35798744›Full record

ArticleNature communications2022

Characterization of core fucosylation via sequential enzymatic treatments of intact glycopeptides and mass spectrometry analysis.

Liwei Cao, T Mamie Lih, Yingwei Hu, Michael Schnaubelt, Shao-Yung Chen, Yangying Zhou, Chuanyu Guo, Mingming Dong, Weiming Yang, Rodrigo Vargas Eguez and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
3.0field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 37 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. A Computational and Chemical Design Strategy for Manipulating Glycan-Protein Recognition.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Article
  11. Article
  12. Article
  13. Mass spectrometry analysis of intact proteinJournal of Zhejiang University. Science. B · 2024
    Article
  14. Article
  15. Article
  16. Article
  17. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 1 institution in 1 country.

Liwei Cao *Department of Pathology, Johns Hopkins University, Baltimore, MD, 21231, USA.
T Mamie Lih *Department of Pathology, Johns Hopkins University, Baltimore, MD, 21231, USA.
Yingwei HuDepartment of Pathology, Johns Hopkins University, Baltimore, MD, 21231, USA.
Michael SchnaubeltDepartment of Pathology, Johns Hopkins University, Baltimore, MD, 21231, USA.
Shao-Yung ChenDepartment of Pathology, Johns Hopkins University, Baltimore, MD, 21231, USA.
Yangying ZhouDepartment of Pathology, Johns Hopkins University, Baltimore, MD, 21231, USA.ORCID 0000-0002-2181-0841
Chuanyu GuoWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.
Mingming DongDepartment of Pathology, Johns Hopkins University, Baltimore, MD, 21231, USA.
Weiming YangDepartment of Pathology, Johns Hopkins University, Baltimore, MD, 21231, USA.
Rodrigo Vargas EguezDepartment of Pathology, Johns Hopkins University, Baltimore, MD, 21231, USA.
Lijun ChenDepartment of Pathology, Johns Hopkins University, Baltimore, MD, 21231, USA.
David J ClarkDepartment of Pathology, Johns Hopkins University, Baltimore, MD, 21231, USA.
Akrit SodhiWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.ORCID 0000-0001-5441-1014
Qing Kay LiDepartment of Pathology, Johns Hopkins University, Baltimore, MD, 21231, USA.
Hui ZhangDepartment of Pathology, Johns Hopkins University, Baltimore, MD, 21231, USA. huizhang@jhu.edu.ORCID 0000-0001-8726-7098
Johns Hopkins University · US

Funding

Glycoprotein biomarkers for the early detection of aggressive prostate cancerU01CA152813 · NCI · JOHNS HOPKINS UNIVERSITY · PI ZHANG, HUI · 2010 to 2021
$6.2M
The Comprehensive Proteome Characterization Center at Johns Hopkins: High Precision Discovery and Confirmation of Genoproteomic TargetsU24CA210985 · NCI · JOHNS HOPKINS UNIVERSITY · PI CHAN, DANIEL WANYUI, ZHANG, HUI · 2016 to 2020
$5.3M
Divergent Roles for Hypoxia-Inducible Factor-1 and -2 in Ischemic Retinal DiseaseR01EY029750 · NEI · JOHNS HOPKINS UNIVERSITY · PI SODHI, AKRIT SINGH · 2019 to 2023
$2.6M
Elucidation of novel anti-angiogenic therapies for the prevention and treatment of neovascular glaucomaR01EY032104 · NEI · UNIVERSITY OF MARYLAND BALTIMORE · PI MONTANER, SILVIA V, SODHI, AKRIT SINGH · 2021 to 2024
$2.3M
NCI NIH HHS U01 CA152813NCI NIH HHS U24 CA210985NEI NIH HHS R01 EY029750
6 · The paper itself

Abstract

Core fucosylation of N-linked glycoproteins has been linked to the functions of glycoproteins in physiological and pathological processes. However, quantitative characterization of core fucosylation remains challenging due to the complexity and heterogeneity of N-linked glycosylation. Here we report a mass spectrometry-based method that employs sequential treatment of intact glycopeptides with enzymes (STAGE) to analyze site-specific core fucosylation of glycoproteins. The STAGE method utilizes Endo F3 followed by PNGase F treatment to generate mass signatures for glycosites that are formerly modified by core fucosylated N-linked glycans. We benchmark the STAGE method and use it to characterize site specific core fucosylation of glycoproteins from human hepatocellular carcinoma and pancreatic ductal adenocarcinoma, resulting in the identification of 1130 and 782 core fucosylated glycosites, respectively. These results indicate that our STAGE method enables quantitative characterization of core fucosylation events from complex protein mixtures, which may benefit our understanding of core fucosylation functions in various diseases.

Indexed as

GlycopeptidesLiver NeoplasmsFucoseGlycoproteinsGlycosylationHumansMass SpectrometryFucoseGlycopeptidesGlycoproteins

Identifiers

PMID35798744
PMCPMC9262967
OpenAlexW4284895787

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.