Evidence map›Paper›PMID 35809574›Full record

ArticleNeuron2022

Dynamin is primed at endocytic sites for ultrafast endocytosis.

Yuuta Imoto, Sumana Raychaudhuri, Ye Ma, Pascal Fenske, Eduardo Sandoval, Kie Itoh, Eva-Maria Blumrich, Hideaki T Matsubayashi, Lauren Mamer, Fereshteh Zarebidaki and 12 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuron, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.

0numbers the graph read from it
0cells of the map it votes in
66citing papers in PubMed
11.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

66 citing papers in PubMed, 103 citations in OpenAlex.

  1. Article
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  17. Synaptobrevin-2 disease variants reveal spatial constraints within the presynaptic active zone.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  18. Ultrastructural membrane dynamics of mouse and human cortical synapses.bioRxiv : the preprint server for biology · 2025
    Article
  19. Review
  20. Article

6 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

22 authors at 5 institutions in 3 countries.

Yuuta ImotoDepartment of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA. Electronic address: yuuta.imoto1@gmail.com.
Sumana RaychaudhuriDepartment of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA.
Ye MaDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
Pascal FenskeInstitute of Neurophysiology, Charité Universitätsmedizin Berlin, Berlin, Germany.
Eduardo SandovalDepartment of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA.
Kie ItohDepartment of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA.
Eva-Maria BlumrichCentre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK; The Muir Maxwell Epilepsy Centre, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK; Simons Initiatives for the Developing Brain, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK.
Hideaki T MatsubayashiDepartment of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA; The Center for Cell Dynamics, Johns Hopkins University, Baltimore, MD 21205, USA.
Lauren MamerInstitute of Neurophysiology, Charité Universitätsmedizin Berlin, Berlin, Germany.
Fereshteh ZarebidakiInstitute of Neurophysiology, Charité Universitätsmedizin Berlin, Berlin, Germany.
Berit Söhl-KielczynskiInstitute of Neurophysiology, Charité Universitätsmedizin Berlin, Berlin, Germany.
Thorsten TrimbuchInstitute of Neurophysiology, Charité Universitätsmedizin Berlin, Berlin, Germany.
Shraddha NayakDepartment of Biochemistry, University of Utah, Salt Lake City, UT 84112-0840, USA.
Janet H IwasaDepartment of Biochemistry, University of Utah, Salt Lake City, UT 84112-0840, USA.
Jian LiuDepartment of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA; Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK.
Bin WuThe Center for Cell Dynamics, Johns Hopkins University, Baltimore, MD 21205, USA; Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Biophysics and Biophysical Chemistry, Johns Hopkins University, Baltimore, MD 21205, USA.
Taekjip HaDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Biophysics and Biophysical Chemistry, Johns Hopkins University, Baltimore, MD 21205, USA; Howard Hughes Medical Institute, Baltimore, MD 21205, USA.
Takanari InoueDepartment of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA; The Center for Cell Dynamics, Johns Hopkins University, Baltimore, MD 21205, USA.
Erik M JorgensenHHMI, Department of Biology, University of Utah, Salt Lake City, UT 84112-0840, USA.
Michael A CousinCentre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK; The Muir Maxwell Epilepsy Centre, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK; Simons Initiatives for the Developing Brain, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK.
Christian RosenmundInstitute of Neurophysiology, Charité Universitätsmedizin Berlin, Berlin, Germany. Electronic address: christian.rosenmund@charite.de.
Shigeki WatanabeDepartment of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA; The Center for Cell Dynamics, Johns Hopkins University, Baltimore, MD 21205, USA; Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, Baltimore, MD 21205, USA. Electronic address: shigeki.watanabe@jhmi.edu.
Johns Hopkins University · USCharité - Universitätsmedizin Berlin · DEUniversity of Utah · USUniversity of Edinburgh · GBHoward Hughes Medical Institute · US

Funding

SYNAPTIC FUNCTION IN THE NEMATODE C. ELEGANSR01NS034307 · NINDS · UNIVERSITY OF UTAH · PI JORGENSEN, ERIK M · 1995 to 2024
$5.8M
SYNAPTIC FUNCTION IN THE NEMATODE C. ELEGANSR37NS034307 · NINDS · UNIVERSITY OF UTAH · PI JORGENSEN, ERIK M · 1999 to 2009
$3.3M
Reviving electron microscopy for synaptic cell biologyDP2NS111133 · NINDS · JOHNS HOPKINS UNIVERSITY · PI WATANABE, SHIGEKI · 2018 to 2018
$2.5M
Spatial and molecular determinants of fusion probability and timingR01NS105810 · NINDS · JOHNS HOPKINS UNIVERSITY · PI WATANABE, SHIGEKI · 2018 to 2022
$1.8M
Howard Hughes Medical InstituteNINDS NIH HHS DP2 NS111133NINDS NIH HHS R01 NS034307NINDS NIH HHS R01 NS105810NINDS NIH HHS R37 NS034307Wellcome TrustWellcome Trust 204954/Z/16/Z
6 · The paper itself

Abstract

Dynamin mediates fission of vesicles from the plasma membrane during endocytosis. Typically, dynamin is recruited from the cytosol to endocytic sites, requiring seconds to tens of seconds. However, ultrafast endocytosis in neurons internalizes vesicles as quickly as 50 ms during synaptic vesicle recycling. Here, we demonstrate that Dynamin 1 is pre-recruited to endocytic sites for ultrafast endocytosis. Specifically, Dynamin 1xA, a splice variant of Dynamin 1, interacts with Syndapin 1 to form molecular condensates on the plasma membrane. Single-particle tracking of Dynamin 1xA molecules confirms the liquid-like property of condensates in vivo. When Dynamin 1xA is mutated to disrupt its interaction with Syndapin 1, the condensates do not form, and consequently, ultrafast endocytosis slows down by 100-fold. Mechanistically, Syndapin 1 acts as an adaptor by binding the plasma membrane and stores Dynamin 1xA at endocytic sites. This cache bypasses the recruitment step and accelerates endocytosis at synapses.

Indexed as

Dynamin ISynaptic VesiclesDynaminsEndocytosisNerve Tissue ProteinsDynamin IDynaminsNerve Tissue ProteinsDyn1xADynaminDynamin splice variantsendocytosisflash-and-freezeliquid condensatesphase separationsynaptic vesicle recyclingSyndapinultrafast endocytosis

Identifiers

PMID35809574
PMCPMC9464723
OpenAlexW4284973934

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.