Evidence map›Paper›PMID 35811308›Full record

ArticleThe Journal of infectious diseases2022

Diagnostic Performance of Loop-Mediated Isothermal Amplification and Ultrasensitive Rapid Diagnostic Tests for Malaria Screening Among Pregnant Women in Kenya.

Aaron M Samuels, Oliver Towett, Brian Seda, Ryan E Wiegand, Kephas Otieno, Miriam Chomba, Naomi Lucchi, Dragan Ljolje, Kammerle Schneider, Patrick G T Walker and 4 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of infectious diseases, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 2 pooled it
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 2 syntheses or guidelines pooled it, 10 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 3 countries.

Aaron M SamuelsMalaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Kisumu, Kenya.ORCID 0000-0002-8355-8186
Oliver TowettCentre for Global Health Research, Kenya Medical Research Institute, Kisumu, Kenya.
Brian SedaCentre for Global Health Research, Kenya Medical Research Institute, Kisumu, Kenya.
Ryan E WiegandDivision of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Kephas OtienoCentre for Global Health Research, Kenya Medical Research Institute, Kisumu, Kenya.
Miriam ChombaCentre for Global Health Research, Kenya Medical Research Institute, Kisumu, Kenya.
Naomi LucchiDivision of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Dragan LjoljeDivision of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Kammerle SchneiderCenter for Malaria Control and Elimination, PATH, Seattle, Washington, USA.
Patrick G T WalkerDepartment of Infectious Disease Epidemiology, Imperial College London, London, United Kingdom.
Titus K KwambaiMalaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Kisumu, Kenya.
Laurence SlutskerCenter for Malaria Control and Elimination, PATH, Seattle, Washington, USA.
Feiko O Ter KuileDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom.
Simon K KariukiCentre for Global Health Research, Kenya Medical Research Institute, Kisumu, Kenya.
Kenya Medical Research Institute · KECenters for Disease Control and Prevention · USLiverpool School of Tropical Medicine · GBProgram for Appropriate Technology in Health · USImperial College London · GB

Funding

CGH CDC HHS U01 GH001646Intramural CDC HHS CC999999Medical Research Council MC_PC_MR/P006914/1Medical Research Council MR/R015600/1
6 · The paper itself

Abstract

backgroundScreen-and-treat strategies with sensitive diagnostic tests may reduce malaria-associated adverse pregnancy outcomes. We conducted a diagnostic accuracy study to evaluate new point-of-care tests to screen pregnant women for malaria at their first antenatal visit in western Kenya.

methodsConsecutively women were tested for Plasmodium infection by expert microscopy, conventional rapid diagnostic test (cRDT), ultra sensitive RDT (usRDT), and loop-mediated isothermal amplification (LAMP). Photoinduced electron-transfer polymerase chain reaction (PET-PCR) served as the reference standard. Diagnostic performance was calculated and modelled at low parasite densities.

resultsBetween May and September 2018, 172 of 482 screened participants (35.7%) were PET-PCR positive. Relative to PET-PCR, expert microscopy was least sensitive (40.1%; 95% confidence interval [CI], 32.7%-47.9%), followed by cRDT (49.4%; 95% CI, 41.7%-57.1), usRDT (54.7%; 95% CI, 46.9%-62.2%), and LAMP (68.6%; 95% CI, 61.1%-75.5%). Test sensitivities were comparable in febrile women (n = 90). Among afebrile women (n = 392), the geometric-mean parasite density was 29 parasites/µL and LAMP (sensitivity = 61.9%) and usRDT (43.2%) detected 1.74 (95% CI, 1.31-2.30) and 1.21 (95% CI, 88-2.21) more infections than cRDT (35.6%). Per our model, tests performed similarly at densities >200 parasites/µL. At 50 parasites/µL, the sensitivities were 45%, 56%, 62%, and 74% with expert microscopy, cRDT, usRDT, and LAMP, respectively.

conclusionsThis first-generation usRDT provided moderate improvement in detecting low-density infections in afebrile pregnant women compared to cRDTs.

Indexed as

MalariaMalaria, FalciparumDiagnostic Tests, RoutineFemaleHumansKenyaMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPlasmodium falciparumPregnancyPregnant PeopleSensitivity and Specificitydiagnostic sensitivity in malaria in pregnancyloop-mediated isothermal amplicification for malaria‌malaria in pregnancymalaria screening at first antenatal care clinic visitultrasensitive rapid diagnostic tests for malaria

Identifiers

PMID35811308
PMCPMC9585193
OpenAlexW4285013418

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.