ArticleMolecular plant pathology2022
The integration host factor regulates multiple virulence pathways in bacterial pathogen Dickeya zeae MS2.
Article in Molecular plant pathology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it, 13 citations in OpenAlex.
- Systematic Discovery of a New Catalogue of Tyrosine-Type Integrases in Bacterial Genomic Islands.Applied and environmental microbiology · 2023Pooled it
- Design of a Novel Diamine Biosensor to Guide the Engineering of Escherichia coli for Cadaverine Overproduction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Spatiotemporal coordination of virulence, metabolism, and stress responses shapes infection dynamics ofApplied and environmental microbiology · 2026Article
- Article
- Loss of essential outer membrane functions causes drug hypersensitization inbioRxiv : the preprint server for biology · 2025Article
- A Lrp/AsnC Family Transcriptional Regulator Lrp Is Essential for the Pathogenicity of Dickeya oryzae.Molecular plant pathology · 2025Article
- The Nucleoid Proteins Fis and IHF Positively Regulate the Gene Expression of Operons Responsible for Producing the Cytotoxins Tilimycin and Tilivalline inInternational journal of microbiology · 2025Article
- The role of integration host factor in biofilm and virulence of high-alcohol-producingMicrobiology spectrum · 2023Article
- Pathogenicity and Transcriptomic Analyses of Two "Microbiology spectrum · 2023Article
- The integration host factor regulates multiple virulence pathways in bacterial pathogen Dickeya zeae MS2.Molecular plant pathology · 2022Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dickeya zeae is an aggressive bacterial phytopathogen that infects a wide range of host plants. It has been reported that integration host factor (IHF), a nucleoid-associated protein consisting of IHFα and IHFβ subunits, regulates gene expression by influencing nucleoid structure and DNA bending. To define the role of IHF in the pathogenesis of D. zeae MS2, we deleted either and both of the IHF subunit encoding genes ihfA and ihfB, which significantly reduced the production of cell wall-degrading enzymes (CWDEs), an unknown novel phytotoxin and the virulence factor-modulating (VFM) quorum-sensing (QS) signal, cell motility, biofilm formation, and thereafter the infection ability towards both potato slices and banana seedlings. To characterize the regulatory pathways of IHF protein associated with virulence, IHF binding sites (consensus sequence 5'-WATCAANNNNTTR-3') were predicted and 272 binding sites were found throughout the genome. The expression of 110 tested genes was affected by IHF. Electrophoretic mobility shift assay (EMSA) showed direct interaction of IhfA protein with the promoters of vfmE, speA, pipR, fis, slyA, prtD, hrpL, hecB, hcp, indA, hdaA, flhD, pilT, gcpJ, arcA, arcB, and lysR. This study clarified the contribution of IHF in the pathogenic process of D. zeae by controlling the production of VFM and putrescine QS signals, phytotoxin, and indigoidine, the luxR-solo system, Fis, SlyA, and FlhD transcriptional regulators, and secretion systems from type I to type VI. Characterization of the regulatory networks of IHF in D. zeae provides a target for prevention and control of plant soft rot disease.
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