Evidence mapPaperPMID 35821438Full record

ArticleCell and tissue research2022

Sestrin2 attenuates renal damage by regulating Hippo pathway in diabetic nephropathy.

Yawei Bian, Chonglin Shi, Shan Song, Lin Mu, Ming Wu, Duojun Qiu, Jiajia Dong, Wei Zhang, Chen Yuan, Dongyun Wang and 3 more

Abstract read
PubMed Publisher
In one paragraph

Article in Cell and tissue research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Review
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Molecular medicine reports · 2024
    Article
  12. Article
  13. Review
  14. Sestrin2 in diabetes and diabetic complications.Frontiers in endocrinology · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Yawei BianDepartment of Pathology, Hebei Medical University, Shijiazhuang, 050017, China.
Chonglin ShiDepartment of Pathology, Hebei Medical University, Shijiazhuang, 050017, China.
Shan SongDepartment of Pathology, Hebei Medical University, Shijiazhuang, 050017, China.
Lin MuDepartment of Pathology, Hebei Medical University, Shijiazhuang, 050017, China.
Ming WuDepartment of Pathology, Hebei Medical University, Shijiazhuang, 050017, China.
Duojun QiuDepartment of Pathology, Hebei Medical University, Shijiazhuang, 050017, China.
Jiajia DongDepartment of Pathology, Hebei Medical University, Shijiazhuang, 050017, China.
Wei ZhangDepartment of Pathology, Hebei Medical University, Shijiazhuang, 050017, China.
Chen YuanDepartment of Pathology, Hebei Medical University, Shijiazhuang, 050017, China.
Dongyun WangDepartment of Pathology, Hebei Medical University, Shijiazhuang, 050017, China.
Zihui ZhouDepartment of Pathology, Hebei Medical University, Shijiazhuang, 050017, China.
Xuan DongDepartment of Pathology, Hebei Medical University, Shijiazhuang, 050017, China.
Yonghong ShiDepartment of Pathology, Hebei Medical University, Shijiazhuang, 050017, China. yonghongshi@163.com.ORCID http://orcid.org/0000-0002-8920-6966
Hebei Medical University · CN

Funding

Local Scientific and Technological Development by the Central Government of China 216Z7703GNational Natural Science Foundation of China 81470966Natural Science Foundation of Hebei Province H2021206144Natural Science Foundation of Hebei Province H2021206356
6 · The paper itself

Abstract

Glomerular mesangial cell proliferation and extracellular matrix accumulation contribute to the progression of diabetic nephropathy (DN). As a conserved stress-inducible protein, sestrin2 (Sesn2) plays critical role in the regulation of oxidative stress, inflammation, autophagy, metabolism, and endoplasmic reticulum stress. In this study, we investigated the role of Sesn2 on renal damage in diabetic kidney using transgenic mice overexpressing Sesn2 and the effect of Sesn2 on mesangial cell proliferation and extracellular matrix accumulation in diabetic conditions and the possible molecular mechanisms involved. Sesn2 overexpression improved renal function and decreased glomerular hypertrophy, albuminuria, mesangial expansion, extracellular matrix accumulation, and TGF-β1 expression, as well as oxidative stress in diabetic mice. In vitro experiments, using human mesangial cells (HMCs), revealed that Sesn2 overexpression inhibited high glucose (HG)-induced proliferation, fibronectin and collagen IV production, and ROS generation. Meanwhile, Sesn2 overexpression restored phosphorylation levels of Lats1 and YAP and inhibited TEAD1 expression. Inhibition of Lats1 accelerated HG-induced proliferation and expression of fibronectin and collagen IV. Verteporfin, an inhibitor of YAP, suppressed HG-induced proliferation and expression of fibronectin and collagen IV. However, Sesn2 overexpression reversed Lats1 deficiency-induced Lats1 and YAP phosphorylation, nuclear expression levels of YAP and TEAD1, and proliferation and fibronectin and collagen IV expressions in HMCs exposed to HG. In addition, antioxidant NAC or tempol treatment promoted phosphorylation of Lats1 and YAP and inhibited TEAD1 expression, proliferation, and fibronectin and collagen IV accumulation in HG-treated HMCs. Taken together, Sesn2 overexpression inhibited mesangial cell proliferation and fibrosis via regulating Hippo pathway in diabetic nephropathy. Induction of Sesn2 may be a potential therapeutic target in diabetic nephropathy.

Indexed as

Diabetes Mellitus, ExperimentalDiabetic NephropathiesAnimalsAntioxidantsCollagen Type IVExtracellular MatrixFibronectinsGlucoseHippo Signaling PathwayHumansKidneyMiceNuclear ProteinsProtein Serine-Threonine KinasesReactive Oxygen SpeciesSestrinsAntioxidantsCollagen Type IVFibronectinsGlucoseNuclear ProteinsProtein Serine-Threonine KinasesReactive Oxygen SpeciesSESN2 protein, humanSestrinsTransforming Growth Factor beta1VerteporfinDiabetic nephropathyHippo pathwayMesangial cellOxidative stressSestrin2

Identifiers

PMID35821438
OpenAlexW4285091559

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.