ArticleMicrobial biotechnology2022
Engineering Saccharomyces cerevisiae-based biosensors for copper detection.
Article in Microbial biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- Engineered Optogenetic Circuits In Yeast with Self-Sustained Outputs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Yeast Biosensors for the Safety of Fermented Beverages.Biosensors · 2026Review
- Transposon engineering ofEngineering in life sciences · 2026Article
- A Bluetooth-Enabled Electrochemical Platform Based onBiosensors · 2025Article
- Betalain Pigments: Isolation and Application as Reagents for Colorimetric Methods and Biosensors.Biosensors · 2025Review
- Design and characterization of allantoin-inducible expression systems in budding yeast.Biotechnology for biofuels and bioproducts · 2025Article
- Minimal aromatic aldehyde reduction (MARE) yeast platform for engineering vanillin production.Biotechnology for biofuels and bioproducts · 2024Article
- Copper-Induced In Vivo Gene Amplification in Budding Yeast.Biodesign research · 2024Article
- Reshaping the yeast galactose regulon via GPCR signaling cascade.Cell reports methods · 2023Article
- Engineering a complex, multiple enzyme-mediated synthesis of natural plant pigments in the silkworm,Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Nanotechnology-Enabled Biosensors: A Review of Fundamentals, Design Principles, Materials, and Applications.Biosensors · 2022Review
- Engineering Saccharomyces cerevisiae-based biosensors for copper detection.Microbial biotechnology · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heavy metals, that is Cu(II), are harmful to the environment. There is an increasing demand to develop inexpensive detection methods for heavy metals. Here, we developed a yeast biosensor with reduced-noise and improved signal output for potential on-site copper ion detection. The copper-sensing circuit was achieved by employing a secondary genetic layer to control the galactose-inducible (GAL) system in Saccharomyces cerevisiae. The reciprocal control of the Gal4 activator and Gal80 repressor under copper-responsive promoters resulted in a low-noise and sensitive yeast biosensor for copper ion detection. Furthermore, we developed a betaxanthin-based colorimetric assay, as well as 2-phenylethanol and styrene-based olfactory outputs for the copper ion detection. Notably, our engineered yeast sensor confers a narrow range switch-like behaviour, which can give a 'yes/no' response when coupled with a betaxanthin-based visual phenotype. Taken together, we envision that the design principle established here might be applicable to develop other sensing systems for various chemical detections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.