Evidence map›Paper›PMID 35836227›Full record

ArticleJournal of translational medicine2022

Identification of ENO1 as a prognostic biomarker and molecular target among ENOs in bladder cancer.

Zhengnan Huang, Yilin Yan, Tengjiao Wang, Zeyi Wang, Jinming Cai, Xiangqian Cao, Chenkai Yang, Fang Zhang, Gang Wu, Bing Shen

Open access · goldAbstract read
In one paragraph

Article in Journal of translational medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 18 citations in OpenAlex.

  1. Transcriptional Reprogramming of Cancer Metabolism:International journal of molecular sciences · 2026
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  6. Role of NAT10-mediated acBMC pulmonary medicine · 2025
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  7. Role of ENO1 and its targeted therapy in tumors.Journal of translational medicine · 2024
    Review
  8. Article
  9. Review
  10. The regulatory roles and clinical significance of glycolysis in tumor.Cancer communications (London, England) · 2024
    Review
  11. Article
  12. Review
  13. BeyondMolecular therapy oncolytics · 2023
    Review
  14. Cancers · 2023
    Article
  15. Article
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Zhengnan Huang *Department of Urology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, 200065, China.
Yilin Yan *Department of Urology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, China.
Tengjiao Wang *Shanghai Key Lab of Cell Engineering, Shanghai, 200433, China.
Zeyi WangDepartment of Urology, Shanghai General Hospital Affiliated to Nanjing Medical University, Shanghai, 200080, China.
Jinming CaiDepartment of Urology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, China.
Xiangqian CaoDepartment of Urology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, China.
Chenkai YangDepartment of Urology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, China.
Fang ZhangDepartment of Urology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200080, China. medego@shsmu.edu.cn.
Gang WuDepartment of Urology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, 200065, China. wg_urologist@163.com.
Bing ShenDepartment of Urology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, 200065, China. urodrshenbing@shsmu.edu.cn.ORCID 0000-0002-0952-2257
Shanghai First People's Hospital · CNTongji University · CNRegenerative Medicine Institute · MXShanghai Jiao Tong University · CNShanghai Medical College of Fudan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEnolase is an essential enzyme in the process of glycolysis and has been implicated in cancer progression. Though dysregulation of ENOs has been reported in multiple cancers, their prognostic value and specific role in bladder cancer (BLCA) remain unclear.

methodsMultiple databases were employed to examine the expression of ENOs in BLCA. The expression of ENO1 was also validated in BLCA cell lines and tissue samples by western blotting and immunohistochemistry. Kaplan-Meier analysis, ROC curve, univariate and multivariate Cox regression were performed to evaluate the predictive capability of the ENO1. Gene ontology (GO) and Gene Set Enrichment Analyses (GSEA) analysis were employed to perform the biological processes enrichment. Function experiments were performed to explore the biological role of ENO1 in BLCA. The correlation of ENO1 with immune cell infiltration was explored by CIBERSORT.

resultsBy analyzing three ENO isoforms in multiple databases, we identified that ENO1 was the only significantly upregulated gene in BLCA. High expression level of ENO1 was further confirmed in BLCA tissue samples. Aberrant ENO1 overexpression was associated with clinicopathological characteristics and unfavorable prognosis. Functional studies demonstrated that ENO1 depletion inhibited cancer cell aggressiveness. Furthermore, the expression level of ENO1 was correlated with the infiltration levels of immune cells and immune-related functions.

conclusionsTaken together, our results indicated that ENO1 might serve as a promising prognostic biomarker for prognosticating prognosis associated with the tumor immune microenvironment, suggesting that ENO1 could be a potential immune-related target against BLCA.

Indexed as

Urinary Bladder NeoplasmsBiomarkers, TumorDNA-Binding ProteinsHumansImmunohistochemistryKaplan-Meier EstimatePhosphopyruvate HydratasePrognosisTumor MicroenvironmentTumor Suppressor ProteinsBiomarkers, TumorDNA-Binding ProteinsENO1 protein, humanPhosphopyruvate HydrataseTumor Suppressor ProteinsBladder cancerEnolaseImmune infiltrationMolecular targetPrognostic biomarker

Identifiers

PMID35836227
PMCPMC9281045
OpenAlexW4285388623

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.