Evidence map›Paper›PMID 35869489›Full record

ArticleBiomaterials research2022

Proteomic, mechanical, and biochemical development of tissue-engineered neocartilage.

Benjamin J Bielajew, Ryan P Donahue, Elliott K Lamkin, Jerry C Hu, Vincent C Hascall, Kyriacos A Athanasiou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Benjamin J Bielajew *Department of Biomedical Engineering, University of California Irvine, Irvine, CA, USA.
Ryan P Donahue *Department of Biomedical Engineering, University of California Irvine, Irvine, CA, USA.
Elliott K LamkinDepartment of Biomedical Engineering, Cleveland Clinic, Cleveland, OH, USA.
Jerry C HuDepartment of Biomedical Engineering, University of California Irvine, Irvine, CA, USA.
Vincent C HascallDepartment of Biomedical Engineering, Cleveland Clinic, Cleveland, OH, USA.
Kyriacos A AthanasiouDepartment of Biomedical Engineering, University of California Irvine, Irvine, CA, USA. athens@uci.edu.ORCID http://orcid.org/0000-0001-5387-8405
University of California, Irvine · USCleveland Clinic · US

Funding

TMJ Disc RegenerationR01DE015038 · NIDCR · UNIVERSITY OF CALIFORNIA-IRVINE · PI ATHANASIOU, KYRIACOS A · 2004 to 2022
$6.1M
Self-assembling process in tissue engineering of articular cartilageR01AR067821 · NIAMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI ATHANASIOU, KYRIACOS A · 2015 to 2019
$1.9M
Engineering biomimetic knee menisci with zonal and anisotropic variationsR01AR071457 · NIAMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI ATHANASIOU, KYRIACOS A · 2017 to 2021
$1.8M
NIAMS NIH HHS R01AR067821NIAMS NIH HHS R01AR071457NIDCR NIH HHS R01DE015038
6 · The paper itself

Abstract

backgroundThe self-assembling process of cartilage tissue engineering is a promising technique to heal cartilage defects, preventing osteoarthritic changes. Given that chondrocytes dedifferentiate when expanded, it is not known if cellular expansion affects the development of self-assembled neocartilage. The objective of this study was to use proteomic, mechanical, and biochemical analyses to quantitatively investigate the development of self-assembled neocartilage derived from passaged, rejuvenated costal chondrocytes.

methodsYucatan minipig costal chondrocytes were used to create self-assembled neocartilage constructs. After 1, 4, 7, 14, 28, 56, or 84 days of self-assembly, constructs were analyzed through a variety of histological, biomechanical, biochemical, and proteomic techniques.

resultsIt was found that temporal trends in neocartilage formation are similar to those seen in native hyaline articular cartilage development. For example, between days 7 and 84 of culture, tensile Young's modulus increased 4.4-times, total collagen increased 2.7-times, DNA content decreased 69.3%, collagen type II increased 1.5-times, and aggrecan dropped 55.3%, mirroring trends shown in native knee cartilage. Importantly, collagen type X, which is associated with cartilage calcification, remained at low levels (≤ 0.05%) at all neocartilage developmental time points, similar to knee cartilage (< 0.01%) and unlike donor rib cartilage (0.98%).

conclusionsIn this work, bottom-up proteomics, a powerful tool to interrogate tissue composition, was used for the first time to quantify and compare the proteome of a developing engineered tissue to a recipient tissue. Furthermore, it was shown that self-assembled, costal chondrocyte-derived neocartilage is suitable for a non-homologous approach in the knee.

Indexed as

CartilageCartilage tissue engineeringDevelopmentKnee cartilageProteomics

Identifiers

PMID35869489
PMCPMC9308280
OpenAlexW4286486505

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.