Evidence map›Paper›PMID 35884331›Full record

ArticleBiosensors2022

Label-Free and Homogeneous Electrochemical Biosensor for Flap Endonuclease 1 Based on the Target-Triggered Difference in Electrostatic Interaction between Molecular Indicators and Electrode Surface.

Jianping Zheng, Xiaolin Xu, Hanning Zhu, Zhipeng Pan, Xianghui Li, Fang Luo, Zhenyu Lin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.0field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 11 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Jianping ZhengDepartment of Oncology, Shengli Clinical Medical College, Fujian Medical University, Fujian Provincial Hospital, Fuzhou 350001, China.
Xiaolin XuDepartment of Clinical Laboratory, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350004, China.
Hanning ZhuDepartment of Clinical Laboratory, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350004, China.
Zhipeng PanDepartment of Clinical Laboratory, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350004, China.
Xianghui LiDepartment of Clinical Laboratory, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350004, China.
Fang LuoMinistry of Education Key Laboratory for Analysis Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, China.
Zhenyu LinMinistry of Education Key Laboratory for Analysis Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, China.ORCID 0000-0001-7890-6812
Fujian Medical University · CNFuzhou University · CN

Funding

National Natural Science Foundation of China 22174018Natural Science Foundation of Fujian Province 2020J01650
6 · The paper itself

Abstract

Target-induced differences in the electrostatic interactions between methylene blue (MB) and indium tin oxide (ITO) electrode surface was firstly employed to develop a homogeneous electrochemical biosensor for flap endonuclease 1 (FEN1) detection. In the absence of FEN1, the positively charged methylene blue (MB) is free in the solution and can diffuse onto the negatively charged ITO electrode surface easily, resulting in an obvious electrochemical signal. Conversely, with the presence of FEN1, a 5'-flap is cleaved from the well-designed flapped dumbbell DNA probe (FDP). The remained DNA fragment forms a closed dumbbell DNA probe to trigger hyperbranched rolling circle amplification (HRCA) reaction, generating plentiful dsDNA sequences. A large amount of MB could be inserted into the produced dsDNA sequences to form MB-dsDNA complexes, which contain a large number of negative charges. Due to the strong electrostatic repulsion between MB-dsDNA complexes and the ITO electrode surface, a significant signal drop occurs. The signal change (Δ

Indexed as

Biosensing TechniquesElectrochemical TechniquesDNADNA ProbesElectrodesFlap EndonucleasesLimit of DetectionMethylene BlueStatic ElectricityDNADNA ProbesFlap EndonucleasesMethylene Blueelectrochemical biosensorflap endonuclease 1homogeneoushyperbranched rolling circle amplificationlabel-free

Identifiers

PMID35884331
PMCPMC9313405
OpenAlexW4285733275

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.