Evidence map›Paper›PMID 35894841›Full record

ArticleOrthopaedic surgery2022

Melatonin Attenuates the Progression of Osteoarthritis in Rats by Inhibiting Inflammation and Related Oxidative Stress on the Surface of Knee Cartilage.

Chenghui Ke, Hongyun Li, Dan Yang, Hao Ying, Hongwen Zhu, Jian Wang, Jun Xu, Lin Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Orthopaedic surgery, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 14 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Review
  6. Melatonin and bone-related diseases: an updated mechanistic overview of current evidence and future prospects.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Chenghui KeDepartment of Orthopaedics, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Hongyun LiDepartment of Anesthesiology, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Dan YangDepartment of Orthopaedics, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Hao YingDepartment of Orthopaedics, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Hongwen ZhuTianjin Hospital, Tianjin Academy of Integrative Medicine, Tianjin, China.
Jian WangTongji University School of Medicine, Shanghai, China.
Jun XuTongji University School of Medicine, Shanghai, China.
Lin WangDepartment of Orthopaedics, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.ORCID https://orcid.org/0000-0001-6078-1214
Shanghai Jiao Tong University · CNTongji University · CNTianjin Hospital · CN

Funding

Medical and industrial cross research Fund of "Star of Jiaotong University" program of Shanghai Jiao Tong University YG2022QN098Medical Innovation Research Project of Shanghai Science and Technology Innovation Action Plan 20Y11913400National Major Science and Technology Infrastructure Project of Translational Medicine, Shanghai Jiao Tong University TMSK-2020-123
6 · The paper itself

Abstract

objectiveTo investigate the correlation between melatonin and osteoarthritis (OA) in rats. To explore the relevant mechanisms in the occurrence and development of osteoarthritis in rats, and to further understand the disease of osteoarthritis.

methodsForty healthy 6-month-old male SD rats were randomly divided into two groups: sham and drug intervention groups. Pre-OA modeling, enzyme-linked immunosorbent assay was employed to detect the levels of IL-1β, IL-6, COX-2, and melatonin in the serum of the rats in each group. For OA modeling, we administered an injection of papain into the knee cavity of all rats. The levels of IL-1β, IL-6, and COX-2 in the serum of rats in each group were detected 2 weeks after the modeling. Additionally, 2 weeks after the modeling, the rats in the drug intervention group were intraperitoneally injected with melatonin antagonists. The rats in the sham group were intraperitoneally injected with normal saline for 2 weeks. The levels of IL-1β, IL-6, and COX-2 in the serum of each group were measured at the second, third, and fourth weeks after the drug intervention, and the levels of melatonin in the serum were measured at the second week after the drug intervention. Finally, the rats were euthanized by cervical dislocation, and pathological sections were collected from the knee joint to observe the pathological tissue changes under a microscope, and Mankin score was determined. The independent samples t-test method was used for analysis.

resultsThe imaging examination after the drug intervention showed that the modeling of knee osteoarthritis in rats was successful. In the pathological findings, HE staining showed a legible cartilage structure of each layer, with cartilage proliferation and partial cartilage tearing to the radial layer. The tide line was intact; toluidine blue staining revealed more obvious changes. The differences among the mean values of IL-6, IL-1β, and COX-2 measured in each period were statistically significant (t = 5.50, p < 0.05). The measured mean values of IL-6, IL-1β, and COX-2 revealed statistically significant differences among the groups (t = 2.01, p < 0.05). The intergroup comparison of the Mankin scores in each period showed statistically significant differences.

conclusionMelatonin may inhibit inflammation and associated oxidative stress on the surface of knee cartilage. It may be related to the repair and regeneration of articular surface cartilage during the development of OA in the rat knee joint.

Indexed as

Cartilage, ArticularMelatoninOsteoarthritis, KneeAnimalsCyclooxygenase 2InflammationInterleukin-6Knee JointMaleOxidative StressPapainRatsRats, Sprague-DawleyCyclooxygenase 2Interleukin-6MelatoninPapainInflammatory factorMelatonin antagonistOsteoarthritis modelPapainSD male rats

Identifiers

PMID35894841
PMCPMC9483081
OpenAlexW4288051170

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.