Evidence map›Paper›PMID 35907922›Full record

ArticleScientific data2022

Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice.

Caroline K David, Yae K Sugimura, Prajkta S Kallurkar, Maria Cristina D Picardo, Margaret S Saha, Gregory D Conradi Smith, Christopher A Del Negro

Open access · goldAbstract readDataset
In one paragraph

Article in Scientific data, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.3field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Role of NaCell reports · 2023
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Caroline K DavidDepartment of Applied Science, William & Mary, 540 Landrum Drive, Williamsburg, Virginia, 23185, USA.ORCID 0000-0003-1136-7476
Yae K SugimuraDepartment of Neuroscience, Jikei University School of Medicine, 3-25-8 Nishi-shimbashi, Minato, Tokyo, 105-8461, Japan.ORCID 0000-0001-8840-797X
Prajkta S KallurkarDepartment of Applied Science, William & Mary, 540 Landrum Drive, Williamsburg, Virginia, 23185, USA.ORCID 0000-0003-2911-6595
Maria Cristina D PicardoDepartment of Applied Science, William & Mary, 540 Landrum Drive, Williamsburg, Virginia, 23185, USA.ORCID 0000-0001-8912-2175
Margaret S SahaDepartment of Biology, William & Mary, 540 Landrum Drive, Williamsburg, Virginia, 23185, USA.ORCID 0000-0003-0096-2667
Gregory D Conradi SmithDepartment of Applied Science, William & Mary, 540 Landrum Drive, Williamsburg, Virginia, 23185, USA.ORCID 0000-0002-1054-6790
Christopher A Del NegroDepartment of Applied Science, William & Mary, 540 Landrum Drive, Williamsburg, Virginia, 23185, USA. cadeln@wm.edu.ORCID 0000-0002-7848-8224
William & Mary · USJikei University School of Medicine · JP

Funding

Neurophysiology of breathing behavior in neonatal mice in vitroR01HL104127 · NHLBI · COLLEGE OF WILLIAM AND MARY · PI DEL NEGRO, CHRISTOPHER A. · 2010 to 2018
$2.6M
Ion Channel Mechanisms of Inspiratory Breathing Movements in MiceR01NS107296 · NINDS · COLLEGE OF WILLIAM AND MARY · PI DEL NEGRO, CHRISTOPHER A. · 2019 to 2022
$1.1M
Molecular Analysis of Early Neural PlasticityR15HD096415 · NICHD · COLLEGE OF WILLIAM AND MARY · PI SAHA, MARGARET S · 2018 to 2018
$402k
NHLBI NIH HHS R01 HL104127NICHD NIH HHS R15 HD096415NINDS NIH HHS R01 NS107296
6 · The paper itself

Abstract

Neurons in the brainstem preBötzinger complex (preBötC) generate the rhythm and rudimentary motor pattern for inspiratory breathing movements. We performed whole-cell patch-clamp recordings from inspiratory neurons in the preBötC of neonatal mouse slices that retain breathing-related rhythmicity in vitro. We classified neurons based on their electrophysiological properties and genetic background, and then aspirated their cellular contents for single-cell RNA sequencing (scRNA-seq). This data set provides the raw nucleotide sequences (FASTQ files) and annotated files of nucleotide sequences mapped to the mouse genome (mm10 from Ensembl), which includes the fragment counts, gene lengths, and fragments per kilobase of transcript per million mapped reads (FPKM). These data reflect the transcriptomes of the neurons that generate the rhythm and pattern for inspiratory breathing movements.

Indexed as

NeuronsRespiratory CenterTranscriptomeAnimalsAnimals, NewbornMicePatch-Clamp TechniquesRespirationSingle-Cell Analysis

Identifiers

PMID35907922
PMCPMC9338969
OpenAlexW4288801636

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.