Evidence map›Paper›PMID 35933378›Full record

ArticleBMC molecular and cell biology2022

Effects of an inhibitor of the SHH signaling pathway on endometrial cells of patients with endometriosis.

Yanan He, J Wang, Xinyan Jiang, Jianhua Gao, Yan Cheng, Tian Liang, Jun Zhou, Liyuan Sun, Guangmei Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC molecular and cell biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Yanan HeDepartment of Gynaecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
J WangDepartment of Gynaecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Xinyan JiangDepartment of Gynaecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Jianhua GaoDepartment of Gynaecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Yan ChengDepartment of Gynaecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Tian LiangDepartment of Gynaecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Jun ZhouDepartment of Gynaecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Liyuan SunDepartment of Gynaecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Guangmei ZhangDepartment of Gynaecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China. guangmeizhang@126.com.
First Affiliated Hospital of Harbin Medical University · CNHarbin Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEndometriosis is one of the most common gynecological diseases, and seriously reduces the quality of life of patients. However, the pathogenesis of this disease is unclear. Therefore, more studies are needed to elucidate its pathogenesis. Our previous publication found that the Sonic Hedgehog (SHH) signaling pathway was activated in endometriosis. This study tested whether SHH signaling in endometrial stromal cells (ESCs) was critical for the pathogenesis of endometriosis.

methodsTo examine the effect of inhibiting the SHH signaling pathway on endometriosis, we first isolated ESCs from eutopic endometrial tissues of patients with or without endometriosis and identified the extracted cells by morphological observation and immunofluorescence. Then, we treated ESCs with the GLI inhibitor GANT61 and used CCK-8, wound healing and invasion assays to detect cell activities, such as proliferation, invasion and metastasis. Furthermore, we detected the expression of key proteins and proliferation markers of the SHH signaling pathway in the lesions of nude mice using immunochemistry.

resultsWe demonstrated that higher concentrations of GANT61 decreased the proliferation rate and migration distance of ESCs. We observed that GANT61 inhibited the invasion of ESCs. In addition, blockage of the SHH signaling pathway significantly reduced cell proliferation in vitro.

conclusionsOur study suggested that inhibition of the SHH pathway is involved in cell proliferation and invasive growth in the pathogenesis of endometriosis.

Indexed as

EndometriosisAnimalsFemaleHedgehog ProteinsHumansMiceMice, NudeQuality of LifeSignal TransductionHedgehog ProteinsSHH protein, humanShh protein, mouseEndometriosisSignaling pathwaySonic hedgehog

Identifiers

PMID35933378
PMCPMC9356504
OpenAlexW4290034024

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.