Evidence map›Paper›PMID 35938989›Full record

ArticleThe Journal of experimental medicine2022

Single-cell analyses reveal early thymic progenitors and pre-B cells in zebrafish.

Sara A Rubin, Chloé S Baron, Cecilia Pessoa Rodrigues, Madeleine Duran, Alexandra F Corbin, Song P Yang, Cole Trapnell, Leonard I Zon

Open access · hybridAbstract read
In one paragraph

Article in The Journal of experimental medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
4.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 53 citations in OpenAlex.

  1. Article
  2. Article
  3. Tissue-Specific Expression of the EWSR1::FLI1 Fusion Protein IdentifiesInternational journal of molecular sciences · 2026
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  16. Thymic Mimetic Cells: Ontogeny as Immunology.Annual review of cell and developmental biology · 2024
    Review
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Sara A RubinStem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital and Dana-Farber Cancer Institute, Boston, MA.ORCID 0000-0002-3484-5461
Chloé S BaronStem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital and Dana-Farber Cancer Institute, Boston, MA.ORCID 0000-0001-8351-4768
Cecilia Pessoa RodriguesStem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital and Dana-Farber Cancer Institute, Boston, MA.ORCID 0000-0001-8617-6565
Madeleine DuranDepartment of Genome Sciences, University of Washington, Seattle, WA.ORCID 0000-0003-1648-2369
Alexandra F CorbinStem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital and Dana-Farber Cancer Institute, Boston, MA.ORCID 0000-0002-1759-885X
Song P YangStem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital and Dana-Farber Cancer Institute, Boston, MA.ORCID 0000-0002-3064-3141
Cole TrapnellDepartment of Genome Sciences, University of Washington, Seattle, WA.ORCID 0000-0002-8105-4347
Leonard I ZonStem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital and Dana-Farber Cancer Institute, Boston, MA.ORCID 0000-0003-0860-926X
Boston Children's Hospital · USUniversity of Washington · US

Funding

Transcriptional and epigenetic heterogeneity of stem/progenitor cellsP01HL131477 · NHLBI · MASSACHUSETTS GENERAL HOSPITAL · PI David T Scadden · 2017 to 2026
$24.6M
PATHOPHYSIOLOGY OF HUMAN BLOOD CELLST32HL007574 · NHLBI · CHILDREN'S HOSPITAL BOSTON · PI Daniel Evan Bauer · 1985 to 2026
$18.4M
Transcriptional response to signaling during hematopoiesisR01HL144780 · NHLBI · BOSTON CHILDREN'S HOSPITAL · PI ZON, LEONARD IRA · 2019 to 2022
$2.1M
Single-Cell Profiling and Lineage Tracing of Zebrafish HematopoiesisF30HL152628 · NHLBI · HARVARD MEDICAL SCHOOL · PI RUBIN, SARA ANN · 2020 to 2024
$195k
NHLBI NIH HHS F30 HL152628NHLBI NIH HHS P01 HL131477NHLBI NIH HHS R01 HL144780NHLBI NIH HHS T32 HL007574
6 · The paper itself

Abstract

The zebrafish has proven to be a valuable model organism for studying hematopoiesis, but relatively little is known about zebrafish immune cell development and functional diversity. Elucidating key aspects of zebrafish lymphocyte development and exploring the breadth of effector functions would provide valuable insight into the evolution of adaptive immunity. We performed single-cell RNA sequencing on ∼70,000 cells from the zebrafish marrow and thymus to establish a gene expression map of zebrafish immune cell development. We uncovered rich cellular diversity in the juvenile and adult zebrafish thymus, elucidated B- and T-cell developmental trajectories, and transcriptionally characterized subsets of hematopoietic stem and progenitor cells and early thymic progenitors. Our analysis permitted the identification of two dendritic-like cell populations and provided evidence in support of the existence of a pre-B cell state. Our results provide critical insights into the landscape of zebrafish immunology and offer a foundation for cellular and genetic studies.

Indexed as

Hematopoietic Stem CellsZebrafishAnimalsHematopoiesisPrecursor Cells, B-LymphoidSingle-Cell AnalysisThymus Gland

Identifiers

PMID35938989
PMCPMC9365674
OpenAlexW4290643168

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.