Evidence map›Paper›PMID 35944004›Full record

SynthesisPloS one2022

Assessing the consistency of iPSC and animal models in cystic fibrosis modelling: A meta-analysis.

Toqa Darwish, Azhar Al-Khulaifi, Menatalla Ali, Rana Mowafy, Abdelilah Arredouani, Suhail A Doi, Mohamed M Emara

Erratum issuedOpen access · goldAbstract readMeta-Analysis
PubMed Full text at PMC Publisher
In one paragraph

Synthesis in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Toqa DarwishBasic Medical Sciences Department, College of Medicine, Qatar University, Doha, Qatar.
Azhar Al-KhulaifiBasic Medical Sciences Department, College of Medicine, Qatar University, Doha, Qatar.
Menatalla AliBasic Medical Sciences Department, College of Medicine, Qatar University, Doha, Qatar.
Rana MowafyBasic Medical Sciences Department, College of Medicine, Qatar University, Doha, Qatar.
Abdelilah ArredouaniDiabetes Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Education City, Qatar Foundation, Doha, Qatar.
Suhail A DoiDepartment of Population Medicine, College of Medicine, QU Health, Qatar University, Doha, Qatar.
Mohamed M EmaraBasic Medical Sciences Department, College of Medicine, Qatar University, Doha, Qatar.ORCID 0000-0002-0921-6313
Qatar University · QAHamad bin Khalifa University · QA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionCystic fibrosis (CF) is a hereditary autosomal recessive disorder caused by a range of mutations in the CF Transmembrane Conductance Regulator (CFTR) gene. This gene encodes the CFTR protein, which acts as a chloride channel activated by cyclic AMP (cAMP). This meta-analysis aimed to compare the responsiveness of induced pluripotent stem cells (iPSCs) to cAMP analogues to that of commonly used animal models.

methodsDatabases searched included PubMed, Scopus, and Medline from inception to January 2020. A total of 8 and 3 studies, respectively, for animal models and iPSCs, were analyzed. Studies were extracted for investigating cAMP-stimulated anion transport by measuring the short circuit current (Isc) of chloride channels in different animal models and iPSC systems We utilized an inverse variance heterogeneity model for synthesis.

resultsOur analysis showed considerable heterogeneity in the mean Isc value in both animal models and iPSCs studies (compared to their WT counterparts), and both suffer from variable responsiveness based on the nature of the underlying model. There was no clear advantage of one over the other.

conclusionsStudies on both animal and iPSCs models generated considerable heterogeneity. Given the potential of iPSC-derived models to study different diseases, we recommend paying more attention to developing reproducible models of iPSC as it has potential if adequately developed.

Indexed as

Cystic FibrosisInduced Pluripotent Stem CellsAnimalsChloride ChannelsCyclic AMPCystic Fibrosis Transmembrane Conductance RegulatorModels, AnimalChloride ChannelsCyclic AMPCystic Fibrosis Transmembrane Conductance Regulator

Identifiers

PMID35944004
PMCPMC9362911
OpenAlexW4290779423

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.