Evidence map›Paper›PMID 35958099›Full record

ArticleMethodsX2022

A new colorimetric method for determining antioxidant levels using 3,5-dibromo-4-nitrosobenzene sulfonate (DBNBS).

Takeki Hamasaki, Taichi Kashiwagi, Takaaki Komatsu, Shigeru Kabayama, Noboru Nakamichi, Kiichiro Teruya, Sanetaka Shirahata

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MethodsX, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Takeki HamasakiDepartment of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 744, Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
Taichi KashiwagiDepartment of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 744, Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
Takaaki KomatsuDepartment of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 744, Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
Shigeru KabayamaNihon Trim Co. Ltd, 1-8-34 Oyodonaka, Kita-ku, Osaka, 531-0076, Japan.
Noboru NakamichiDepartment of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 744, Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
Kiichiro TeruyaDepartment of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 744, Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
Sanetaka ShirahataDepartment of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, 744, Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
Kyushu University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We describe here a novel assay that determines the total a+ntioxidative activities of known antioxidants and antioxidants in beverages. The method employs the substrate 3,5-dibromo-4-nitrosobenzene sulfonate (DBNBS) that yields the colored product 3,5,3',5'-tetrabromoazobenzene sulfate sodium salt (azo-TBBS). The amounts of azo-TBBS are measured using HPLC and then used to calculate total antioxidative capacity (TAC) values. We first show that the TAC values measured using the new DBNBS system were significantly higher compared with the control. The assay was validated through further analysis of 56 compounds, including previously characterized antioxidants. The data are consistent with published values. Here we describe in detail the application of the DBNBS method to the measurement of the TAC values of eight beverages, including wines and fruit juices. The DBNBS assay employs a readily applicable protocol that sensitively determines the levels of antioxidants in foodstuffs. - A new DBNBS-mediated antioxidant assay system is compared with standard DPPH and ORAC assays - DBNBS traps hydrogen radicals to generate a readily measured colored reduction product that quantifies antioxidant levels.

Indexed as

AntioxidantsAntioxidative activityHydrogen atom transferTotal antioxidant capacity

Identifiers

PMID35958099
PMCPMC9358457
OpenAlexW4288078146

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.