Evidence map›Paper›PMID 35972144›Full record

ArticlemBio2022

Barcoding Genetically Distinct Plasmodium falciparum Strains for Comparative Assessment of Fitness and Antimalarial Drug Resistance.

Manuela Carrasquilla, Ndey F Drammeh, Mukul Rawat, Theo Sanderson, Zenon Zenonos, Julian C Rayner, Marcus C S Lee

Open access · goldAbstract read
In one paragraph

Article in mBio, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
4.0field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 21 citations in OpenAlex.

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  14. Novel Inhibitors ofJournal of medicinal chemistry · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 3 countries.

Manuela CarrasquillaWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United Kingdom.ORCID 0000-0002-7953-4376
Ndey F DrammehWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United Kingdom.
Mukul RawatWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United Kingdom.
Theo SandersonWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United Kingdom.
Zenon ZenonosWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United Kingdom.
Julian C RaynerWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United Kingdom.
Marcus C S LeeWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, United Kingdom.ORCID 0000-0002-4973-0915
Wellcome Sanger Institute · GBAstraZeneca (United Kingdom) · GBThe Francis Crick Institute · GBUniversity of Cambridge · GB

Funding

Wellcome Trust 206194/Z/17/ZWellcome Trust 210918/Z/18/Z
6 · The paper itself

Abstract

The repeated emergence of antimalarial drug resistance in Plasmodium falciparum, including to the current frontline antimalarial artemisinin, is a perennial problem for malaria control. Next-generation sequencing has greatly accelerated the identification of polymorphisms in resistance-associated genes but has also highlighted the need for more sensitive and accurate laboratory tools to profile current and future antimalarials and to quantify the impact of drug resistance acquisition on parasite fitness. The interplay of fitness and drug response is of fundamental importance in understanding why particular genetic backgrounds are better at driving the evolution of drug resistance in natural populations, but the impact of parasite fitness landscapes on the epidemiology of drug resistance has typically been laborious to accurately quantify in the lab, with assays being limited in accuracy and throughput. Here we present a scalable method to profile fitness and drug response of genetically distinct P. falciparum strains with well-described sensitivities to several antimalarials. We leverage CRISPR/Cas9 genome-editing and barcode sequencing to track unique barcodes integrated into a nonessential gene (

Indexed as

AntimalarialsArtemisininsPlasmodium falciparumComplex MixturesDrug ResistanceGenetic FitnessProtozoan ProteinsAntimalarialsArtemisininsComplex MixturesProtozoan Proteinsapicomplexan parasitesdrug resistancefitnessmalaria

Identifiers

PMID35972144
PMCPMC9600763
OpenAlexW4292185274

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.