Evidence mapPaperPMID 35976278Full record

ArticleActa cirurgica brasileira2022

Genistein improves mitochondrial function and inflammatory in rats with diabetic nephropathy via inhibiting MAPK/NF-κB pathway.

Ying Li, Santao Ou, Qi Liu, Linwang Gan, Liling Zhang, Yujie Wang, Jianhua Qin, Jin Liu, Weihua Wu

Open access · goldAbstract read
In one paragraph

Article in Acta cirurgica brasileira, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
9.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 26 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Ying LiMD. SiChuan Clinical Research Center for Nephropathy - Affiliated Hospital of Southwest Medical University - Department of Nephrology - Luzhou, China.ORCID http://orcid.org/0000-0001-9612-3379
Santao OuMD. SiChuan Clinical Research Center for Nephropathy - Affiliated Hospital of Southwest Medical University - Department of Nephrology - Luzhou, China.ORCID http://orcid.org/0000-0003-2806-4025
Qi LiuMD. SiChuan Clinical Research Center for Nephropathy - Affiliated Hospital of Southwest Medical University - Department of Nephrology - Luzhou, China.ORCID http://orcid.org/0000-0003-3840-3537
Linwang GanMD. SiChuan Clinical Research Center for Nephropathy - Affiliated Hospital of Southwest Medical University - Department of Nephrology - Luzhou, China.ORCID http://orcid.org/0000-0002-8255-0393
Liling ZhangMD. SiChuan Clinical Research Center for Nephropathy - Affiliated Hospital of Southwest Medical University - Department of Nephrology - Luzhou, China.ORCID http://orcid.org/0000-0002-5538-4473
Yujie WangMD. SiChuan Clinical Research Center for Nephropathy - Affiliated Hospital of Southwest Medical University - Department of Nephrology - Luzhou, China.ORCID http://orcid.org/0000-0002-9325-7926
Jianhua QinMD. SiChuan Clinical Research Center for Nephropathy - Affiliated Hospital of Southwest Medical University - Department of Nephrology - Luzhou, China.ORCID http://orcid.org/0000-0001-6691-3441
Jin LiuMD. SiChuan Clinical Research Center for Nephropathy - Affiliated Hospital of Southwest Medical University - Department of Nephrology - Luzhou, China.ORCID http://orcid.org/0000-0002-9701-8754
Weihua WuMD. SiChuan Clinical Research Center for Nephropathy - Affiliated Hospital of Southwest Medical University - Department of Nephrology - Luzhou, China.ORCID http://orcid.org/0000-0002-9717-8010
Southwest Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo investigate the effect of genistein on inflammation and mitochondrial function of diabetic nephropathy.

methodsDiabetic nephropathy model was established in Sprague-Dawley rats. Automatic biochemical analyzer was employed to detect the kidney function index, serum creatinine, serum urea nitrogen, and 24 h-urine protein and blood glucose. Hematoxylin and eosin staining and periodic acid Schiff staining were used to observe renal morphology. Mitochondrial changes and podocyte integrity were monitored by transmission electron microscope. The expression levels of mfn2, NOX4, P53, MAPK, and NF-κB were detected by Western blotting. The changes of mitochondrial membrane potential were measured by JC-1. The level of mfn2 was assessed by immunofluorescence assay.

resultsGenistein ameliorated the kidney function with reduced Scr and blood glucose. The expressions of NOX4, MAPK, p65 and p53 were downregulated, while the expression of mnf2 was the opposite in genistein-treated kidneys. Further investigations revealed that genistein reduced expansion of mesangial matrix and oxidative stress, protected podocyte integrity and increased mitochondrial membrane potential.

conclusionsGenistein could alleviate diabetic nephropathy through inhibiting MAPK/NF-κB pathway, improving mitochondrial function and anti-inflammatory.

Indexed as

Diabetes Mellitus, ExperimentalDiabetic NephropathiesAnimalsBlood GlucoseGenisteinKidneyMAP Kinase Signaling SystemMitochondriaNF-kappa BRatsRats, Sprague-DawleyTumor Suppressor Protein p53Blood GlucoseGenisteinNF-kappa BTumor Suppressor Protein p53

Identifiers

PMID35976278
PMCPMC9377651
OpenAlexW4291567075

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.