ArticleDevelopment (Cambridge, England)2022
Paternally inherited H3K27me3 affects chromatin accessibility in mouse embryos produced by round spermatid injection.
Article in Development (Cambridge, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Higher-order genome architecture of spermatids guides chromatin assembly in mature sperm.Research square · 2026Article
- A simple, efficient, and scalable method to generate oocyte-like cells in vitro.Life science alliance · 2026Article
- Spermatocyte injection into meiotic oocytes rescues diplotene, but not pachytene, arrest in azoospermic mutant mice.Human reproduction open · 2025Article
- 17α-Ethynylestradiol alters testicular epigenetic profiles and histone-to-protamine exchange in mice.Reproductive biology and endocrinology : RB&E · 2024Article
- Kdm4d mutant mice show impaired sperm motility and subfertility.The Journal of reproduction and development · 2024Article
- Epigenetic priming in the male germline.Current opinion in genetics & development · 2024Review
- Chromatin structure in totipotent mouse early preimplantation embryos.The Journal of reproduction and development · 2024Review
- The Hypothalamic Epigenetic Landscape in Dietary Obesity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- KDM6A/UTX promotes spermatogenic gene expression across generations and is not required for male fertility†.Biology of reproduction · 2024Article
- A systematic scientometric review of paternal inheritance of acquired metabolic traits.BMC biology · 2023Review
- Molecular mechanisms underlying totipotency.Life science alliance · 2023Review
- Polycomb protein SCML2 mediates paternal epigenetic inheritance through sperm chromatin.Nucleic acids research · 2023Article
- Nucleosomes in mammalian sperm: conveying paternal epigenetic inheritance or subject to reprogramming between generations?Current opinion in genetics & development · 2023Review
- Production of mouse offspring from zygotes fertilized with freeze-dried spermatids.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 2 countries.
Funding
Abstract
Round spermatid injection (ROSI) results in a lower birth rate than intracytoplasmic sperm injection, which has hampered its clinical application. Inefficient development of ROSI embryos has been attributed to epigenetic abnormalities. However, the chromatin-based mechanism that underpins the low birth rate in ROSI remains to be determined. Here, we show that a repressive histone mark, H3K27me3, persists from mouse round spermatids into zygotes in ROSI and that round spermatid-derived H3K27me3 is associated with less accessible chromatin and impaired gene expression in ROSI embryos. These loci are initially marked by H3K27me3 but undergo histone modification remodelling in spermiogenesis, resulting in reduced H3K27me3 in normal spermatozoa. Therefore, the absence of epigenetic remodelling, presumably mediated by histone turnover during spermiogenesis, leads to dysregulation of chromatin accessibility and transcription in ROSI embryos. Thus, our results unveil a molecular logic, in which chromatin states in round spermatids impinge on chromatin accessibility and transcription in ROSI embryos, highlighting the importance of epigenetic remodelling during spermiogenesis in successful reproduction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.