Evidence map›Paper›PMID 36004992›Full record

ArticleBiosensors2022

Probing the Hepatitis B Virus E-Antigen with a Nanopore Sensor Based on Collisional Events Analysis.

Ioana C Bucataru, Isabela Dragomir, Alina Asandei, Ana-Maria Pantazica, Alina Ghionescu, Norica Branza-Nichita, Yoonkyung Park, Tudor Luchian

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Ioana C BucataruDepartment of Physics, Alexandru I. Cuza University, 700506 Iasi, Romania.
Isabela DragomirInterdisciplinary Research Institute, Sciences Department, Alexandru I. Cuza University, 700506 Iasi, Romania.
Alina AsandeiInterdisciplinary Research Institute, Sciences Department, Alexandru I. Cuza University, 700506 Iasi, Romania.ORCID 0000-0002-4596-241X
Ana-Maria PantazicaViral Glycoproteins Department, Institute of Biochemistry of the Romanian Academy, 060031 Bucharest, Romania.
Alina GhionescuViral Glycoproteins Department, Institute of Biochemistry of the Romanian Academy, 060031 Bucharest, Romania.
Norica Branza-NichitaViral Glycoproteins Department, Institute of Biochemistry of the Romanian Academy, 060031 Bucharest, Romania.
Yoonkyung ParkDepartment of Biomedical Science and Research Center for Proteinaceous Materials (RCPM), Chosun University, Gwangju 61452, Korea.ORCID 0000-0002-8717-3080
Tudor LuchianDepartment of Physics, Alexandru I. Cuza University, 700506 Iasi, Romania.ORCID 0000-0002-9388-7266
Alexandru Ioan Cuza University · ROInstitute of Biochemistry · ROChosun University · KR

Funding

Unitatea Executiva Pentru Finantarea Invatamantului Superior a Cercetarii Dezvoltarii si Inovarii PN-III-P4-ID-PCE-2020-0011
6 · The paper itself

Abstract

Real-time monitoring, simple operation, and cheaper methods for detecting immunological proteins hold the potential for a solid influence on proteomics and human biology, as they can promote the onset of timely diagnoses and adequate treatment protocols. In this work we present an exploratory study suggesting the applicability of resistive-pulse sensing technology in conjunction with the α-hemolysin (α-HL) protein nanopore, for the detection of the chronic hepatitis B virus (HBV) e-antigen (HBeAg). In this approach, the recognition between HBeAg and a purified monoclonal hepatitis B e antibody (Ab(HBeAg)) was detected via transient ionic current spikes generated by partial occlusions of the α-HL nanopore by protein aggregates electrophoretically driven toward the nanopore's vestibule entrance. Despite the steric hindrance precluding antigen, antibody, or antigen-antibody complex capture inside the nanopore, their stochastic bumping with the nanopore generated clear transient blockade events. The subsequent analysis suggested the detection of protein subpopulations in solution, rendering the approach a potentially valuable label-free platform for the sensitive, submicromolar-scale screening of HBeAg targets.

Indexed as

Hepatitis B, ChronicNanoporesHepatitis B e AntigensHepatitis B virusHumansImmunoglobulinsHepatitis B e AntigensImmunoglobulinsantigenelectrophysiologyhepatitis Bmonoclonal antibodynanoporesingle molecule detection

Identifiers

PMID36004992
PMCPMC9405897
OpenAlexW4289985942

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.