ArticleJournal of cardiovascular development and disease2022
Electroporation Parameters for Human Cardiomyocyte Ablation In Vitro.
Article in Journal of cardiovascular development and disease, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Physiological Tissue Response to Pulsed-Field Ablation for Atrial Fibrillation: Insights from Biomarker Kinetics.Physiological research · 2026Article
- Evaluation of pulsed field ablation lesion characteristics using an in vitro vegetable model.Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing · 2026Review
- Evaluation of variable inter-pulse delay and timing on pulsed field ablation lesion characteristics.Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing · 2026Article
- Evolution of Energy Sources in Surgical Ablation of Atrial Fibrillation: Current Technology and Future Directions.Reviews in cardiovascular medicine · 2026Review
- Role of Computational Modelling in Enhancing Thermal Safety During Cardiac Ablation.Interdisciplinary cardiovascular and thoracic surgery · 2025Review
- Biophysics and Clinical Effectiveness of Irreversible Electroporation for Catheter Ablation of Atrial Fibrillation.Journal of cardiovascular development and disease · 2025Review
- Pulsed Field Ablation: A Review of Preclinical and Clinical Studies.Bioengineering (Basel, Switzerland) · 2025Review
- Pulsed Field Ablation of Atrial Fibrillation: A Novel Technology for Safer and Faster Ablation.Biomedicines · 2024Review
- Pulsed Field Ablation for Atrial Fibrillation: Mechanisms, Advantages, and Limitations.Reviews in cardiovascular medicine · 2024Review
- Opportunities and Challenges in Catheter-Based Irreversible Electroporation for Ventricular Tachycardia.Pathophysiology : the official journal of the International Society for Pathophysiology · 2024Review
- In Vitro Models for Improved Therapeutic Interventions in Atrial Fibrillation.Journal of personalized medicine · 2023Review
- Pearls and Pitfalls of Pulsed Field Ablation.Korean circulation journal · 2023Review
- New Perspectives on Risk Stratification and Treatment in Patients with Atrial Fibrillation: An Analysis of Recent Contributions on theJournal of cardiovascular development and disease · 2023Article
- Comparative Analysis of Temperature Rise between Convective Heat Transfer Method and Computational Fluid Dynamics Method in an Anatomy-Based Left Atrium Model during Pulsed Field Ablation: A Computational Study.Journal of cardiovascular development and disease · 2023Article
- Human cardiomyocytes are more susceptible to irreversible electroporation by pulsed electric field than human esophageal cells.Physiological reports · 2022Article
- Effect of Anisotropic Electrical Conductivity Induced by Fiber Orientation on Ablation Characteristics of Pulsed Field Ablation in Atrial Fibrillation Treatment: A Computational Study.Journal of cardiovascular development and disease · 2022Article
- HumanFrontiers in physiology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Cardiac ablation with irreversible electroporation (IRE) is quickly being established as a modality of choice for atrial fibrillation treatment. While it has not yet been optimised, IRE has the potential to significantly limit collateral damage and improve cell-specific targeting associated with other energy sources. However, more tissue and cell-specific evidence is required to demonstrate the selective threshold parameters for human cells. The aim here is to determine the optimal ablation threshold parameters related to lesion size for human cardiomyocytes in 2D culture. Conventional biphasic pulses of different field strengths and on-times were delivered in a monolayer culture system of human AC16 cardiomyocytes. The dynamics of cell death and lesion dimensions were examined at different time points. Human cardiomyocytes are susceptible to significant electroporation and cell death at a field strength of 750 V/cm or higher with 100 μs pulses. Increasing the IRE on-time from 3 ms to 60 ms reduces the effective field threshold to 250 V/cm. Using very short pulses of 2 μs and 5 μs also causes significant cell death, but only at fields higher than 1000 V/cm. A longer on-time results in more cell death and induced greater lesion area in 2D models. In addition, different forms of cell death are predicted based on the evolution of cell death over time. This study presents important findings on the ability of different IRE parameters to induce human cardiomyocyte cell death. Lesion size can be tuned by appropriate choice of IRE parameters and cardiomyocytes display an upregulation of delayed cell death 24 h after electroporation, which is an important consideration for clinical practice.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.