ArticlePathogens (Basel, Switzerland)2022
Hydrophobic Alpha-Helical Short Peptides in Overlapping Reading Frames of the Coronavirus Genome.
Takashi Okura, Kazuya Shirato, Masatoshi Kakizaki, Satoko Sugimoto, Shutoku Matsuyama, Tomohisa Tanaka, Yohei Kume, Mina Chishiki, Takashi Ono, Kohji Moriishi and 5 more
Open access · goldAbstract read
In one paragraphArticle in Pathogens (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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0citing papers in PubMed
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What it found
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5 · Who and what moneyAuthors and funding
15 authors at 5 institutions in 1 country.
Takashi OkuraDepartment of Virology 3, National Institute of Infectious Diseases, Musashimurayama 208-0011, Tokyo, Japan.
Kazuya ShiratoDepartment of Virology 3, National Institute of Infectious Diseases, Musashimurayama 208-0011, Tokyo, Japan.ORCID 0000-0002-3375-9799 Masatoshi KakizakiDepartment of Virology 3, National Institute of Infectious Diseases, Musashimurayama 208-0011, Tokyo, Japan.
Satoko SugimotoDepartment of Virology 3, National Institute of Infectious Diseases, Musashimurayama 208-0011, Tokyo, Japan.
Shutoku MatsuyamaCenter for Influenza and Respiratory Virus Research, National Institute of Infectious Diseases, Musashimurayama 208-0011, Tokyo, Japan.
Tomohisa TanakaDepartment of Microbiology, Faculty of Medicine, Graduate Faculty of Interdisciplinary Research, University of Yamanashi, Chuo 409-3898, Yamanashi, Japan.ORCID 0000-0002-6343-0230 Yohei KumeDepartment of Pediatrics, School of Medicine, Fukushima Medical University, Fukushima 960-1295, Fukushima, Japan.ORCID 0000-0003-2985-1282 Mina ChishikiDepartment of Pediatrics, School of Medicine, Fukushima Medical University, Fukushima 960-1295, Fukushima, Japan.ORCID 0000-0002-0785-7053 Takashi OnoDepartment of Pediatrics, School of Medicine, Fukushima Medical University, Fukushima 960-1295, Fukushima, Japan.
Kohji MoriishiDepartment of Microbiology, Faculty of Medicine, Graduate Faculty of Interdisciplinary Research, University of Yamanashi, Chuo 409-3898, Yamanashi, Japan.ORCID 0000-0002-9542-5188 Masashi SonoyamaDivision of Molecular Science, Graduate School of Science and Technology, Gunma University, Kiryu 376-8515, Gunma, Japan.ORCID 0000-0003-3674-5170 Mitsuaki HosoyaDepartment of Pediatrics, School of Medicine, Fukushima Medical University, Fukushima 960-1295, Fukushima, Japan.ORCID 0000-0003-1626-3625 Koichi HashimotoDepartment of Pediatrics, School of Medicine, Fukushima Medical University, Fukushima 960-1295, Fukushima, Japan.ORCID 0000-0001-7186-7603 Katsumi MaenakaLaboratory of Biomolecular Science, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Hokkaido, Japan.ORCID 0000-0002-5459-521X Makoto TakedaDepartment of Virology 3, National Institute of Infectious Diseases, Musashimurayama 208-0011, Tokyo, Japan.ORCID 0000-0002-8194-7727 National Institute of Infectious Diseases · JPFukushima Medical University · JPHokkaido University · JPGunma University · JPUniversity of Yamanashi · JP
Funding
Japanese Respiratory Foundation NAMHLW 20HA2007MHLW 21HA2003Research Foundation for Opto-Science and Technology NAThe Naito Foundation NA
6 · The paper itselfAbstract
In this study, we show that the coronavirus (CoV) genome may encode many functional hydrophobic alpha-helical peptides (HAHPs) in overlapping reading frames of major coronaviral proteins throughout the entire viral genome. These HAHPs can theoretically be expressed from non-canonical sub-genomic (sg)RNAs that are synthesized in substantial amounts in infected cells. We selected and analyzed five and six HAHPs encoded in the S gene regions of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and Middle East respiratory syndrome coronavirus (MERS-CoV), respectively. Two and three HAHPs derived from SARS-CoV-2 and MERS-CoV, respectively, specifically interacted with both the SARS-CoV-2 and MERS-CoV S proteins and inhibited their membrane fusion activity. Furthermore, one of the SARS-CoV-2 HAHPs specifically inhibited viral RNA synthesis by accumulating at the site of viral RNA synthesis. Our data show that a group of HAHPs in the coronaviral genome potentially has a regulatory role in viral propagation.
Indexed as
alpha-helixcoronavirusnidovirusoverlapping frameSARS-CoV-2sub-genomic RNA
Identifiers
PMID36014999
PMCPMC9415614
OpenAlexW4289731659
What Socratic holds
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LicenceCC BY
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