Evidence map›Paper›PMID 36063142›Full record

ArticleJournal of extracellular vesicles2022

Necroptosis is associated with Rab27-independent expulsion of extracellular vesicles containing RIPK3 and MLKL.

Kartik Gupta, Kyle A Brown, Marvin L Hsieh, Brandon M Hoover, Jianxin Wang, Mitri K Khoury, Vijaya Satish Sekhar Pilli, Reagan S H Beyer, Nihal R Voruganti, Sahil Chaudhary and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of extracellular vesicles, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
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  5. Article
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  7. Article
  8. Review
  9. Review
  10. Exocytosis of Nanoparticles: A Comprehensive Review.Nanomaterials (Basel, Switzerland) · 2023
    Review
  11. Review
  12. Review
  13. Review
  14. Article
  15. Necroptosis in CNS diseases: Focus on astrocytes.Frontiers in aging neuroscience · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 1 country.

Kartik GuptaDivision of Vascular Surgery, Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
Kyle A BrownDivision of Vascular Surgery, Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
Marvin L HsiehDivision of Vascular Surgery, Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
Brandon M HooverDepartment of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
Jianxin WangWisconsin Center for NanoBioSystems, School of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
Mitri K KhouryDivision of Vascular Surgery, Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
Vijaya Satish Sekhar PilliDivision of Vascular Surgery, Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
Reagan S H BeyerDivision of Vascular Surgery, Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
Nihal R VorugantiDivision of Vascular Surgery, Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
Sahil ChaudharyDivision of Vascular Surgery, Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
David S RobertsDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
Regina M MurphyDepartment of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
Seungpyo HongWisconsin Center for NanoBioSystems, School of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
Ying GeDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
Bo LiuDivision of Vascular Surgery, Department of Surgery, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.
University of Wisconsin–Madison · USUniversity of Wisconsin System · US

Funding

Enabling Top-Down Proteomics through Materials Chemistry and NanotechnologyR01GM117058 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING, JIN, SONG · 2015 to 2023
$2.7M
Vascular Surgery Research Training ProgramT32HL110853 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI MATSUMURA, JON STEVEN · 2012 to 2021
$2.6M
Ultra High Resolution Mass Spectrometer for Biomedical ResearchS10OD018475 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING · 2015 to 2015
$2.0M
Role of RIP3-laden extracellular vesicles in thrombosis and aortic aneurysmR01HL149404 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI LIU, BO · 2020 to 2023
$2.0M
MASH Explorer, a Comprehensive Software Environment for Top-Down ProteomicsR01GM125085 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING · 2018 to 2021
$1.2M
NHLBI NIH HHS R01 HL149404NHLBI NIH HHS T32 HL110853NIGMS NIH HHS R01 GM117058NIGMS NIH HHS R01 GM125085NIH HHS S10 OD018475
6 · The paper itself

Abstract

Extracellular vesicle (EV) secretion is an important mechanism used by cells to release biomolecules. A common necroptosis effector-mixed lineage kinase domain like (MLKL)-was recently found to participate in the biogenesis of small and large EVs independent of its function in necroptosis. The objective of the current study is to gain mechanistic insights into EV biogenesis during necroptosis. Assessing EV number by nanoparticle tracking analysis revealed an increased number of EVs released during necroptosis. To evaluate the nature of such vesicles, we performed a newly adapted, highly sensitive mass spectrometry-based proteomics on EVs released by healthy or necroptotic cells. Compared to EVs released by healthy cells, EVs released during necroptosis contained a markedly higher number of unique proteins. Receptor interacting protein kinase-3 (RIPK3) and MLKL were among the proteins enriched in EVs released during necroptosis. Further, mouse embryonic fibroblasts (MEFs) derived from mice deficient of Rab27a and Rab27b showed diminished basal EV release but responded to necroptosis with enhanced EV biogenesis as the wildtype MEFs. In contrast, necroptosis-associated EVs were sensitive to Ca

Indexed as

Extracellular VesiclesNecroptosisAnimalsFibroblastsMicePhosphorylationProtein KinasesReceptor-Interacting Protein Serine-Threonine KinasesMLKL protein, mouseProtein KinasesReceptor-Interacting Protein Serine-Threonine KinasesRipk3 protein, mouselysosomal exocytosisMLKLnecroptosisproteomicsRIPK3SEVs

Identifiers

PMID36063142
PMCPMC9443950
OpenAlexW4294671766

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.