Evidence map›Paper›PMID 36066602›Full record

ArticlePlant cell reports2022

Abscisic acid induces the expression of AsKIN during the recovery period of garlic cryopreservation.

Xiaodong Xing, Min Liu, Fangling Jiang, Rong Zhou, Yunhe Bai, Hanyu Wei, Deng Zhang, Jingjing Wei, Zhen Wu

Abstract read
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In one paragraph

Article in Plant cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 2 countries.

Xiaodong XingCollege of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
Min LiuCollege of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
Fangling JiangCollege of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
Rong ZhouCollege of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
Yunhe BaiCollege of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
Hanyu WeiCollege of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
Deng ZhangCollege of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
Jingjing WeiCollege of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
Zhen WuCollege of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China. zpzxyy@163.com.ORCID http://orcid.org/0000-0002-8243-3613
Nanjing Agricultural University · CN

Funding

Fundamental Research Funds for the Central Universities KJFP201702National Natural Science Foundation of China 31372056National Natural Science Foundation of China 31872125Priority Academic Program Development of Jiangsu Higher Education Institutions PAPD
6 · The paper itself

Abstract

key messageAbscisic acid induced the expression of AsKIN during the recovery period of garlic cryopreservation. AsKIN was identified as a gene involved in cold and osmotic stress resistance. Cryopreservation has been proven to be effective in removing viruses from garlic. However, oxidative damage in cryopreservation has a significant impact on the survival after preservation. Abscisic acid (ABA) has been shown to reduce oxidative stress and promote the survival after cryopreservation. However, it is not clear which genes play important roles in this process. In this study, we added ABA to the dehydration step and analyzed the transcriptomic divergences between the ABA-treated group and the control group in three cryogenic steps (dehydration, unloading and recovery). By short time-series expression miner (STEM) analysis and weighted gene co-expression network analysis (WGCNA), the recovery step was identified as the period of significant changes in gene expression levels in cryopreservation. The addition of ABA promoted the upregulated expression of microtubule-related genes in the recovery step. We further identified AsKIN as a hub gene in the recovery step and verified its function. The results showed that overexpression of AsKIN enhanced the tolerance of Arabidopsis to oxidative stress in cryopreservation, influenced the expression of genes in response to cold and osmotic stress and promoted plant growth after stress. The AsKIN gene is likely to be involved in the plant response to cold stress and osmotic stress. These results reveal the molecular mechanisms of ABA in cryopreservation and elucidate the potential biological functions of the kinesin-14 subfamily.

Indexed as

ArabidopsisArabidopsis ProteinsGarlicAbscisic AcidAntioxidantsCryopreservationDehydrationGene Expression Regulation, PlantKinesinsAbscisic AcidAntioxidantsArabidopsis ProteinsKinesinsAbscisic acidAsKINCryopreservationGarlicRNA-Seq

Identifiers

PMID36066602
OpenAlexW4294712455

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.