Evidence map›Paper›PMID 36067404›Full record

ArticleGut microbes

Deoxycholic acid induces gastric intestinal metaplasia by activating STAT3 signaling and disturbing gastric bile acids metabolism and microbiota.

Duochen Jin, Keting Huang, Miao Xu, Hongjin Hua, Feng Ye, Jin Yan, Guoxin Zhang, Yun Wang

Open access · goldAbstract read
In one paragraph

Article in Gut microbes. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.

0numbers the graph read from it
0cells of the map it votes in
58citing papers in PubMed
19.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

58 citing papers in PubMed, 101 citations in OpenAlex.

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  12. Signaling pathways and targeted interventions for precancers.Signal transduction and targeted therapy · 2026
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  17. Diet-induced dampness-heat psoriasis is characterized by reducedFrontiers in cellular and infection microbiology · 2026
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Duochen JinDepartment of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, Nanjing China.
Keting HuangDepartment of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, Nanjing China.
Miao XuDepartment of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, Nanjing China.
Hongjin HuaDepartment of Pathology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Feng YeDepartment of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, Nanjing China.
Jin YanDepartment of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, Nanjing China.
Guoxin ZhangDepartment of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, Nanjing China.
Yun WangDepartment of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, Nanjing China.
Jiangsu Province Hospital · CNNanjing Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intestinal metaplasia (IM) is the inevitable precancerous stage to develop intestinal-type gastric cancer (GC). Deoxycholic acid (DCA) is the main bile acid (BA) component of duodenogastric reflux and has shown an increased concentration during the transition from chronic gastritis to IM associated with continued STAT3 activation. However, the mechanisms underlying how DCA facilitates IM in the gastric epithelium need exploration. We evaluated IM and bile reflux in corpus tissues from 161 subjects undergoing GC screening. Cell survival and proliferation, proinflammatory cytokine expression and TGR5/STAT3/KLF5 axis activity were measured in normal human gastric cells, cancer cells, and organoid lines derived from C57BL/6, FVB/N and insulin-gastrin (INS-GAS) mice treated with DCA. The effects of DCA on IM development were determined in INS-GAS mice with long-term DCA supplementation, after which the gastric bacterial and BA metabolic profiles were measured by 16S rRNA gene sequencing and LC-MS. We revealed a BA-triggered TGR5/STAT3/KLF5 pathway in human gastric IM tissues. In gastric epithelial cells, DCA promoted proliferation and apoptotic resistance, upregulated proinflammatory cytokines and IM markers, and facilitated STAT3 phosphorylation, nuclear accumulation and DNA binding to the KLF5 promoter. DCA triggered STAT3 signaling and the downstream IM marker KLF5 in mouse gastric organoids

Indexed as

Gastrointestinal MicrobiomeMicrobiotaPrecancerous ConditionsAnimalsBile Acids and SaltsDeoxycholic AcidHumansMetaplasiaMiceMice, Inbred C57BLRNA, Ribosomal, 16SSTAT3 Transcription FactorBile Acids and SaltsDeoxycholic AcidRNA, Ribosomal, 16SSTAT3 protein, humanSTAT3 Transcription Factorbile acids metabolic profilingcarcinogenesisDeoxycholic acidgastric intestinal metaplasiagastric microbiotaKLF5STAT3TGR5

Identifiers

PMID36067404
PMCPMC9467587
OpenAlexW4294811690

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.