Evidence mapPaperPMID 36083191Full record

ArticleAngewandte Chemie (International ed. in English)2022

Activity-based Photoacoustic Probes Reveal Elevated Intestinal MGL and FAAH Activity in a Murine Model of Obesity.

Melissa Y Lucero, Sarah H Gardner, Anuj K Yadav, Austin Borri, Zhenxiang Zhao, Jefferson Chan

Open access · hybridAbstract read
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 27 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Melissa Y LuceroDepartment of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL61801, USA.ORCID 0000-0002-7221-1144
Sarah H GardnerDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL61801, USA.ORCID 0000-0002-9356-7190
Anuj K YadavDepartment of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL61801, USA.ORCID 0000-0002-4736-4392
Austin BorriDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL61801, USA.
Zhenxiang ZhaoDepartment of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL61801, USA.ORCID 0000-0002-7431-5174
Jefferson ChanDepartment of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL61801, USA.ORCID 0000-0003-4139-4379
University of Illinois Urbana-Champaign · US

Funding

Chemical Probes for Studying Stem Cells and Environmental Interactions in AgingR35GM133581 · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · 2025 to 2025
$412k
NIGMS NIH HHS R35 GM133581
6 · The paper itself

Abstract

Obesity is a chronic health condition characterized by the accumulation of excessive body fat which can lead to and exacerbate cardiovascular disease, type-II diabetes, high blood pressure, and cancer through systemic inflammation. Unfortunately, visualizing key mediators of the inflammatory response, such as monoacylglycerol lipase (MGL) and fatty acid amide hydrolase (FAAH), in a selective manner is a profound challenge owing to an overlapping substrate scope that involves arachidonic acid (AA). Specifically, these enzymes work in concert to generate AA, which in the context of obesity, has been implicated to control appetite and energy metabolism. In this study, we developed the first selective activity-based sensing probes to detect MGL (PA-HD-MGL) and FAAH (PA-HD-FAAH) activity via photoacoustic imaging. Activation of PA-HD-MGL and PA-HD-FAAH by their target enzymes resulted in 1.74-fold and 1.59-fold signal enhancements, respectively. Due to their exceptional selectivity profiles and deep-tissue photoacoustic imaging capabilities, these probes were employed to measure MGL and FAAH activity in a murine model of obesity. Contrary to conflicting reports suggesting levels of MGL can be attenuated or elevated, our results support the latter. Indeed, we discovered a marked increase of both targets in the gastrointestinal tract. These key findings set the stage to uncover the role of the endocannabinoid pathway in obesity-mediated inflammation.

Indexed as

EndocannabinoidsMonoacylglycerol LipasesAmidohydrolasesAnimalsArachidonic AcidDisease Models, AnimalHumansInflammationMiceObesityAmidohydrolasesArachidonic AcidEndocannabinoidsMonoacylglycerol LipasesActivity-Based SensingEndocannabinoid SystemInflammationObesityPhotoacoustic Imaging

Identifiers

PMID36083191
PMCPMC9613605
OpenAlexW4295049453

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.