Evidence map›Paper›PMID 36084321›Full record

ArticleDiabetes2022

Ca2+ Oscillations, Waves, and Networks in Islets From Human Donors With and Without Type 2 Diabetes.

Marko Gosak, Richard Yan-Do, Haopeng Lin, Patrick E MacDonald, Andraž Stožer

Open access · bronzeAbstract read
In one paragraph

Article in Diabetes, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
7.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 34 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 3 countries.

Marko GosakInstitute of Physiology, Faculty of Medicine, University of Maribor, Maribor, Slovenia.
Richard Yan-DoHong Kong Centre for Cerebro-Cardiovascular Health Engineering, Hong Kong Science Park, Department of Biomedical Engineering, City University of Hong Kong, Kowloon, Hong Kong.
Haopeng LinDepartment of Pharmacology and Alberta Diabetes Institute, University of Alberta, Edmonton, Canada.
Patrick E MacDonaldDepartment of Pharmacology and Alberta Diabetes Institute, University of Alberta, Edmonton, Canada.ORCID 0000-0002-5439-6288
Andraž StožerInstitute of Physiology, Faculty of Medicine, University of Maribor, Maribor, Slovenia.
University of Alberta · CAUniversity of Maribor · SI

Funding

Linking islet cell function and identity from in vitro to in situU01DK120447 · NIDDK · UNIVERSITY OF ALBERTA · PI ARROJO E DRIGO, RAFAEL, LUNDBERG, EMMA · 2018 to 2021
$3.0M
NIDDK NIH HHS U01 DK120447
6 · The paper itself

Abstract

Pancreatic islets are highly interconnected structures that produce pulses of insulin and other hormones, maintaining normal homeostasis of glucose and other nutrients. Normal stimulus-secretion and intercellular coupling are essential to regulated secretory responses, and these hallmarks are known to be altered in diabetes. In the current study, we used calcium imaging of isolated human islets to assess their collective behavior. The activity occurred in the form of calcium oscillations, was synchronized across different regions of islets through calcium waves, and was glucose dependent: higher glucose enhanced the activity, elicited a greater proportion of global calcium waves, and led to denser and less fragmented functional networks. Hub regions were identified in stimulatory conditions, and they were characterized by long active times. Moreover, calcium waves were found to be initiated in different subregions and the roles of initiators and hubs did not overlap. In type 2 diabetes, glucose dependence was retained, but reduced activity, locally restricted waves, and more segregated networks were detected compared with control islets. Interestingly, hub regions seemed to suffer the most by losing a disproportionately large fraction of connections. These changes affected islets from donors with diabetes in a heterogeneous manner.

Indexed as

Diabetes Mellitus, Type 2Islets of LangerhansCalciumGlucoseHumansInsulinCalciumGlucoseInsulin

Identifiers

PMID36084321
PMCPMC9750953
OpenAlexW4295038126

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.