Evidence map›Paper›PMID 36100620›Full record

ArticleScientific reports2022

Longitudinal investigation of a xenograft tumor zebrafish model using polarization-sensitive optical coherence tomography.

Antonia Lichtenegger, Junya Tamaoki, Roxane Licandro, Tomoko Mori, Pradipta Mukherjee, Lixuan Bian, Lisa Greutter, Shuichi Makita, Adelheid Wöhrer, Satoshi Matsusaka and 3 more

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
0.6field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it, 6 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 3 countries.

Antonia LichteneggerComputational Optics Group, University of Tsukuba, Tsukuba, Japan. antonia.lichtenegger@cog-labs.org.
Junya TamaokiDepartment of Molecular and Developmental Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
Roxane LicandroComputational Imaging Research Lab, Department of Biomedical Imaging and Image-guided Therapy, Medical University of Vienna, Vienna, Austria.
Tomoko MoriClinical Research and Regional Innovation, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
Pradipta MukherjeeComputational Optics Group, University of Tsukuba, Tsukuba, Japan.
Lixuan BianDepartment of Molecular and Developmental Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
Lisa GreutterDivision of Neuropathology and Neurochemistry, Department of Neurology, Medical University of Vienna, Vienna, Austria.
Shuichi MakitaComputational Optics Group, University of Tsukuba, Tsukuba, Japan.
Adelheid WöhrerDivision of Neuropathology and Neurochemistry, Department of Neurology, Medical University of Vienna, Vienna, Austria.
Satoshi MatsusakaClinical Research and Regional Innovation, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
Makoto KobayashiDepartment of Molecular and Developmental Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
Bernhard BaumannCenter for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Austria.
Yoshiaki YasunoComputational Optics Group, University of Tsukuba, Tsukuba, Japan.
University of Tsukuba · JPMedical University of Vienna · AT

Funding

Brainstem Arousal Network in Human Consciousness: Healthy development vs SIDSR01HD102616 · NICHD · MASSACHUSETTS GENERAL HOSPITAL · PI Lilla Zollei · 2021 to 2026
$3.5M
Austrian Science Fund FWF J 4460Austrian Science Fund FWF Schrödinger grant J4460European Research Council ERC StG 640396 OPTIMALZNICHD NIH HHS 1R01HD102616-01A1NICHD NIH HHS R01 HD102616
6 · The paper itself

Abstract

Breast cancer is a leading cause of death in female patients worldwide. Further research is needed to get a deeper insight into the mechanisms involved in the development of this devastating disease and to find new therapy strategies. The zebrafish is an established animal model, especially in the field of oncology, which has shown to be a promising candidate for pre-clinical research and precision-based medicine. To investigate cancer growth in vivo in zebrafish, one approach is to explore xenograft tumor models. In this article, we present the investigation of a juvenile xenograft zebrafish model using a Jones matrix optical coherence tomography (JM-OCT) prototype. Immunosuppressed wild-type fish at 1-month post-fertilization were injected with human breast cancer cells and control animals with phosphate buffered saline in the tail musculature. In a longitudinal study, the scatter, polarization, and vasculature changes over time were investigated and quantified in control versus tumor injected animals. A significant decrease in birefringence and an increase in scattering signal was detected in tumor injected zebrafish in comparison to the control once. This work shows the potential of JM-OCT as a non-invasive, label-free, three-dimensional, high-resolution, and tissue-specific imaging tool in pre-clinical cancer research based on juvenile zebrafish models.

Indexed as

Breast NeoplasmsTomography, Optical CoherenceAnimalsDisease Models, AnimalFemaleHeterograftsHumansLongitudinal StudiesZebrafish

Identifiers

PMID36100620
PMCPMC9470556
OpenAlexW4295421528

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.