Evidence map›Paper›PMID 36133331›Full record

ArticleNanoscale advances2022

Immuno-chromatic probe based lateral flow assay for point-of-care detection of Japanese encephalitis virus NS1 protein biomarker in clinical samples using a smartphone-based approach.

Akanksha Roberts, Drishya Prakashan, Himani Dhanze, Ravi Kumar Gandham, Sonu Gandhi, G Taru Sharma

Open access · goldAbstract read
In one paragraph

Article in Nanoscale advances, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
7.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 38 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Review
  6. An Integrated Approach to Improve the Assay Performance of Quantum Dot-Based Lateral Flow Immunoassays by Using Silver Deposition.Microchemical journal : devoted to the application of microtechniques in all branches of science · 2023
    Article
  7. Review
  8. Article
  9. Review
  10. Review
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Akanksha RobertsDBT-National Institute of Animal Biotechnology (NIAB) Hyderabad-500032 Telangana India gandhi@niab.org.in +91-040-23120127.ORCID https://orcid.org/0000-0001-5978-8582
Drishya PrakashanDBT-National Institute of Animal Biotechnology (NIAB) Hyderabad-500032 Telangana India gandhi@niab.org.in +91-040-23120127.
Himani DhanzeICAR-Indian Veterinary Research Institute (IVRI) Bareilly-243122 Uttar Pradesh India.
Ravi Kumar GandhamICAR-Indian Veterinary Research Institute (IVRI) Bareilly-243122 Uttar Pradesh India.
Sonu GandhiDBT-National Institute of Animal Biotechnology (NIAB) Hyderabad-500032 Telangana India gandhi@niab.org.in +91-040-23120127.ORCID https://orcid.org/0000-0003-0960-2780
G Taru SharmaDBT-National Institute of Animal Biotechnology (NIAB) Hyderabad-500032 Telangana India gandhi@niab.org.in +91-040-23120127.
National Institute of Animal Biotechnology · INIndian Veterinary Research Institute · IN

Funding

NIEHS NIH HHS 27302C0038
6 · The paper itself

Abstract

Lateral flow assays (LFAs) are one of the most economical, point-of-care (PoC) diagnostic assays that exploit the colorimetric properties of gold nanoparticles (AuNPs). Up to the best of our knowledge, no rapid antigen-based LFA exists for Japanese Encephalitis Virus (JEV) detection. Herein, we have reported a novel portable sandwich-type LFA for on-site detection of the non-structural 1 (NS1) secretory protein of JEV. In-house JEV NS1 antibodies (Abs) were generated and labelled with AuNPs as immunoprobes. A glass fibre membrane conjugate pad was soaked with AuNPs-Ab solution, while the JEV NS1 Ab and anti-rabbit IgG 2° Ab were coated as the test and control lines, respectively, on a nitrocellulose (NC) membrane. Different layers of the LFA were fabricated and various parameters were standardised for optimum colour intensity development. JEV negative serum samples spiked with JEV NS1 Ags (linear range - 1 pg ml

Identifiers

PMID36133331
PMCPMC9470087
OpenAlexW4293384787

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.