Evidence map›Paper›PMID 36202914›Full record

ArticleScientific reports2022

Altered functional responses by PAR1 agonist in murine dextran sodium sulphate-treated colon.

Tae Sik Sung, Suk Bae Moon, Brian A Perrino, Kenton M Sanders, Sang Don Koh

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Tae Sik SungDepartment of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV, 89557, USA.
Suk Bae MoonDepartment of Surgery, Kangwon National University School of Medicine, Chuncheon, 24341, South Korea.
Brian A PerrinoDepartment of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV, 89557, USA.
Kenton M SandersDepartment of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV, 89557, USA.
Sang Don KohDepartment of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV, 89557, USA. skoh@med.unr.edu.
University of Nevada, Reno · USKangwon National University · KRSeoul National University Hospital · KR

Funding

Tissue, Cell and Tissue Culture CoreP01DK041315 · NIDDK · UNIVERSITY OF NEVADA RENO · PI SANDERS, KENTON M · 1989 to 2018
$26.3M
NIDDK NIH HHS 41315NIDDK NIH HHS P01 DK041315
6 · The paper itself

Abstract

Protease-activated receptor-1 (PAR1) is highly expressed in murine colonic smooth muscles. Responses to PAR1 activation are complex and result from responses in multiple cell types. We investigated whether PAR1 responses are altered in inflamed colon induced by dextran sodium sulfate (DSS)-treatment. Colitis was induced in C57BL/6 mice by administration of 3% DSS in drinking water for 7 days. Measurements of isometric force, transmembrane potentials from impaled smooth muscle cells, quantitative PCR and Western blots were performed. Thrombin, an activator of PAR1, caused transient hyperpolarization and relaxation of untreated colons, but these responses decreased in DSS-treated colons. Apamin caused depolarization and increased contractions of muscles from untreated mice. This response was decreased in DSS-treated colons. Expression of Kcnn3 and Pdgfra also decreased in DSS-treated muscles. A second phase of thrombin responses is depolarization and increased contractions in untreated muscles. However, thrombin did cause depolarization in DSS-treated colon, yet it increased colonic contractions. The latter effect was associated with enhanced expression of MYPT1 and CPI-17. The propagation velocity and frequency of colonic migrating motor complexes in DSS-treated colon was significantly higher compared to control colons. In summary, DSS treatment causes loss of transient relaxations due to downregulation of SK3 channels in PDGFRα

Indexed as

ColitisDrinking WaterAnimalsApaminColonDextran SulfateDisease Models, AnimalMiceMice, Inbred C57BLReceptor, PAR-1Receptor, Platelet-Derived Growth Factor alphaSmall-Conductance Calcium-Activated Potassium ChannelsSulfatesThrombinApaminDextran SulfateDrinking WaterKcnn3 protein, mouseReceptor, PAR-1Receptor, Platelet-Derived Growth Factor alphaSmall-Conductance Calcium-Activated Potassium Channelssodium sulfateSulfatesThrombin

Identifiers

PMID36202914
PMCPMC9537183
OpenAlexW4302299479

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.